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  6. 5'-DMT-rC (Bz)-Suc-CPG Column

5'-DMT-rC (Bz)-Suc-CPG Column

5'-DMT-rC (Bz)-Suc-CPG Column
5'-DMT-rC (Bz)-Suc-CPG Column
5'-DMT-rC (Bz)-Suc-CPG Column
5'-DMT-rC (Bz)-Suc-CPG Column
5'-DMT-rC (Bz)-Suc-CPG Column
5'-DMT-rC (Bz)-Suc-CPG Column

5'-DMT-rC (Bz)-Suc-CPG Column

CPG column for incorporation of unmodified ribo-C at 3' end of an oligonucleotide.

Key features

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  • CPG has aminopropyl succinyl linker
  • Standard luer or Supercolumn pipette column types available
  • Available in different synthesis scales and pore sizes
Option 1: Select a Pore Size
Option 2: Select a Column Type
Option 3: Select a Scale
TBD
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Product information

Various RNA CPG column formats are available, and compatible with a range of instruments. 

Synthesizer
Column
Type/Description
Notes
MerMade 6,12
MerMade, syringe (all scales)
Pipette type column
A MerMade column is also known as a Supercolumn
MerMade 4, 48X, 96E, 192E, 192X
MerMade, Syringe (up to 1.3 mL)
Pipette type column
A MerMade column is also known as a Supercolumn
ABI 384 / 394
Luer
Barrel column with luer fitting at either end
Also known as ALL-FIT or Standard
Expedite 8909
Luer
Barrel column with luer fitting at either end
Also known as ALL-FIT or Standard
ABI3900
MerMade
Pipette type column
A MerMade column is also known as a Supercolumn
K&A H4, H8, H8SE, H2, H32, H64
Luer
Barrel column with luer fitting at either end
Also known as ALL-FIT or Standard
K&A S4CL/S8CL
Luer
  Barrel column with luer fitting at either end 
 Also known as Standard. For this instrument, we recommend the Luer (Standard) column as the ALL-FIT columns have a wider barrel.
Dr Oligo 48
MerMade
Pipette type column
A MerMade column is also known as a Supercolumn
 Dr Oligo 192XLc, 768XLc just plates 
 MerMade, Syringe (up to 1.3 mL) 
Pipette type column
A MerMade column is also known as a Supercolumn
 OligoMaker X12, 48, 192, X192, X96 
MerMade, Syringe (up to 1.3 mL)
Pipette type column
A MerMade column is also known as a Supercolumn

Properties:

  • Appearance: White Powder

Product usage:

  • Cleavage conditions: Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites.We do not recommend using AMA to cleave or deprotect sequences that contain uridine. The AMA may degrade the uridine nucleobase.
  • Deprotection conditions: When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.
  • The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 305.18

Storage and handling:

  • Shipping conditions: Ambient
  • Storage conditions: -15 to -30 °C in sealed container

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