BAC-Optimised Electrocompetent Cells
Clone, construct libraries, transform Gibson Assembly® reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and challenging applications.
Clone, construct libraries, transform Gibson Assembly® reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and challenging applications.
Clone, construct libraries, transform Gibson Assembly® reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and challenging applications.
Stabilise toxic sequences behind T7 or lac-based promoters with this strain containing a LacI repressor, providing tight control over leaky expression.
Clone large inserts in standard plasmids, avoid T1 and T5 phage contamination, and control copy number of CopyControl vectors.
Induce high-level protein expression from T7 promoters with tight control over leaky expression, using this strain containing a LacI repressor.
Induce plasmid copy number in CopyCutter EPI400 strain, for high plasmid yields.
High efficiency F-control strain for phage display applications.
Express difficult or toxic proteins using standard E. coli T7 expression vectors using these unique strains. Also available in chemically competent format.
Construct larger, more complex phage display libraries with highest efficiency cells. SS320 is a non-amber suppressor strain used for phage display.
Perform plasmid rescue for transposition mutants created with the EZ-Tn5 Transposome Kits.
Clone and subclone with high efficiency, chemically competent DH5-alpha cells.
Create large phage display libraries with high efficiency cells recommended for peptide display.
Clone unstable and toxic sequences in standard plasmids, with high efficiency.
Clone large inserts in standard plasmids, and control copy number of CopyControl vectors. EPI300 Electrocompetent cells are recommended for Gibson Assembly® reactions.
Get better colony counts for routine protein expression with any E. coli T7 expression vector, with this high-efficiency BL21(DE3) strain.
Clone, construct libraries, transform Gibson Assembly® reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and challenging applications.
Clone and sub-clone with high-efficiency, recA, endA competent cells.
Eliminate endotoxin at the source with this unique engineered E. coli strain for low-endotoxin protein expression.
LGC, Biosearch Technologies offers the best performance, value, and convenience in competent cells for cloning and expression. Our chemically competent cells offer an unmatched value for routine cloning, and our electrocompetent cells provide the highest possible efficiency for difficult cloning and library construction.
See our Custom Competent Cell Services page to learn how you can receive the high-quality cells in the exact format you need, when you need them. Custom dispensing is available in tubes or 96-well plates, for your strain or ours.
Our competent cells must pass strict QC specifications for transformation efficiency and antibiotic resistance.
Every lot of cells is tested to ensure the cells meet our guaranteed transformation efficiencies. Cells are also tested for resistance to a panel of antibiotics by plating mock-transformed cells on antibiotic plates. Common antibiotic panel includes: kanamycin, carbenicillin (ampicillin), streptomycin, tetracycline, gentamycin and chloramphenicol.
Chemically competent cells | Electrocompetent cells |
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Europe, Middle East, and Africa | |
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UK | +44 1992 470 757 |
Germany | +49 30 5304 2200 |
North America, Latin America | |
Wisconsin, USA | +1 888 575 9695 |
Asia Pacific | |
China | +8621-22509000 |