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NxGen phi29 DNA Polymerase

Supplied at 10,000 U/mL in 10 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.5% Tween-20, 0.5% NP-40, 50% glycerol, pH 7.4 @ 25°C. Also supplied is 10X phi29 DNA Polymerase Buffer composed of 500 mM Tris-HCl, 100 mM (NH4)2SO4, 40 mM Dithiothreitol, 100 mM MgCl2, pH 7.5 @ 25°C. 10,000 U is supplied as 5 × 2,000 U.

NxGen phi29 DNA Polymerase

Excellent strand displacement, fidelity and speed.
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Product information

phi29 DNA Polymerase is a highly processive DNA polymerase with a powerful strand displacement activity and a 3′→ 5′ proofreading exonuclease function. The enzyme is capable of up to 70,000 insertions per binding event.

Unit Definition: 1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTP's into acid insoluble material in 10 minutes at 30 °C.

Source: A recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29.

Unit Concentration 10,000 U/mL
Purity (SDS-PAGE) >99%
SS Exonuclease Functional
E. coli 16S rDNA Contamination 100 U <10 copies
Endonuclease 100 U <10% converted
Storage -20&°C

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