Low Endoxin Cells
ClearColi BL21(DE3) Electrocompetent Cells
Eliminate endotoxin at the source with this unique engineered E. coli strain for low-endotoxin protein expression.
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TG1 Electrocompetent Cells
Product Details
ShowCompetent Cells include Control DNA and Recovery Medium and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
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TG1 Electrocompetent Cells
Construct larger, more complex phage display libraries with highest efficiency cells. This amber suppressor strain can be used for phage display and protein expression.
Key features
Show- Perfect for antibody phage display library creation
- The highest efficiency TG1 competent cells available.
- Create larger libraries; speed discovery
- Bulk custom dispensing available with all of our phage display cells
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Product information
TG1 Electrocompetent Cells. An amber suppressor strain (supE) prepared as highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for phage display library screening. These cells are used for phage display and protein expression
Figure 1. Transformation efficiency of electrocompetent bacterial cells for phage display compared to competitor's specification.
Table 1. Competent Cells for Phage Display libraries
Cell Lines |
Transformation Efficiency |
Cloning Methylated DNA |
BAC, Cosmid Cloning |
Blue/White Screening |
|
TG1 Electrocompetent |
>4 × 1010 |
YES |
NO |
YES |
|
SS320 (MC1061 F') Electrocompetent |
>4× 1010 |
YES |
NO |
YES |
|
MC1061 F-Electrocompetent |
>4× 1010 |
YES |
NO |
YES |
|
ER2738 Electrocompetent |
>2 × 1010 |
YES |
NO |
YES |
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Genotype
- TG1: [F' traD36 proAB lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK - mK -)
- SS320 (MC1061F'): F'[proAB lacIqZ ΔM15 Tn10 (TetR)] araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1
- ER2738: [F'proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10 (tetr)] fhuA2 glnVΔ(lac-proAB) thi-1Δ(hsdS-mcrB)5
- MC1061 F-: F-, araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1
SDS
Manuals and user guides
Product information sheets
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