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RNase A

RNase A

Efficiently degrade unwanted RNA in DNA preparations and other samples.
  • Remove unwanted single-stranded RNA from multiple sample types or reactions.
  • Quick: Rapidly degrade single-stranded RNA with this highly effective endoribonuclease
  • Robust: RNase A is a durable enzyme and resistant to many harsh treatments (e.g. boiling)

Size: 2 mL

Item ID MRNA092
TBD
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Product information

Ribonuclease A (RNase A) is an endoribonuclease that cleaves single-stranded RNA at the 3´ end of pyrimidine residues, forming oligoribonucleotides having 3´-terminal pyrimidine-3´-phosphates. Pyrimidine-3´-monophosphates are also released by RNase A cleavage of adjacent pyrimidine nucleotides. Modified RNA containing pyrimidine-2´-fluoro-dNMPs, such as DuraScript® RNA made by in vitro transcription using the DuraScribe T7 & SP6 Transcription Kits is completely resistant to cleavage by RNase A.

Applications

  • Removal of RNA from DNA preparations.
  • Removal of unhybridised regions of RNA from DNA-RNA or RNA-RNA hybrids.

Composition and Concentration: Provided on the basis of weight at 5 mg/mL in Storage Buffer.

Storage Buffer: 50% glycerol containing 25 mM sodium acetate (pH 4.6).

Quality Control: RNase A is free of contaminating exo- and endodeoxyribonuclease activities.

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