Skip to content Skip to navigation menu

Rec J Exonuclease

Includes 10X Reaction Buffer.

Rec J Exonuclease

Magnesium-dependent exonuclease for 5' to 3' digestion of single-stranded DNA.
  • Specific: Digests ssDNA in the 5' to 3' direction
  • Heat Inactivated: Heat at 65 °C for 20 minutes to kill enzyme activity
  • Flexible: Use to remove primers from completed PCR or to degrade single-stranded linear DNA in dsDNA and plasmid preps

Size: 250 units

Item ID RJ411250
TBD
Add to basket to request a quote

Product information

Rec J Exonuclease, derived from E. coli, catalyses degradation of ssDNA in a 5´ to 3´ direction. Its activity is dependent on Mg2+. Rec J Exonuclease can be heat-inactivated by incubation at 65 °C for 20 minutes.

Applications

  • Removal of primers from completed PCR.
  • Degradation of single-stranded linear DNA in dsDNA and plasmid preps.

Unit Definition: One unit of Rec J Exonuclease catalyses the release of 1 nmol of acid-soluble nucleotides from activated single-stranded calf thymus DNA in 30 minutes at 37 °C under standard assay conditions.

Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 0.1 M NaCl, and 0.1% Triton® X-100.

10X Reaction Buffer: 330 mM Tris-acetate (pH 7.5), 660 mM potassium acetate, 100 mM magnesium, acetate, and 5.0 mM DTT.

Quality Control: Rec J Exonuclease is free of detectable RNase and contaminating DNA endonuclease activities.

SDS

Manuals and user guides

Product information sheets

Access support

Need some support with placing an order, setting up an account, or finding the right protocol?

Contact us