Large or Difficult Cloning
HI-Control 10G Chemically Competent Cells
Product Details
ShowCompetent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
You are currently seeing list prices, to see your prices please log in
HI-Control 10G Chemically Competent Cells
Stabilise toxic sequences behind T7 or lac-based promoters with this strain containing a LacI repressor, providing tight control over leaky expression.
Key features
Show- Reduce leaky expression: The LacI repressor protein binds to the lacO operator, blocking transcription of downstream genes. In the absence of an induction agent such as lactose sugar or IPTG, target protein expression from the adjacent promoter is greatly reduced.
- Obtain high protein yields: Both 10G and BL21(DE3)-based strains express high levels of protein. For expression from a T7 promoter, select the HI-Control BL21(DE3) strain. Other lac-based promoters like T5-lac, tac, trc, and lac, can be used in either HI-Control strain.
- Achieve high efficiency transformations: Highly efficient competent cells give excellent results from small amounts of plasmid DNA.
Size: 12 reactions (SOLOs)
Added to basket
Similar Products
Protein Expression
HI-Control BL21(DE3) Chemically Competent Cells
Induce high-level protein expression from T7 promoters with tight control over leaky expression, using this strain containing a LacI repressor.
Protein Expression
OverExpress Chemically Competent Cells
Express difficult or toxic proteins using standard E. coli T7 expression vectors using these unique strains. Some strains also available in electrocompetent format.
Large or Difficult Cloning
CopyCutter EPI400 Competent Cells
Clone unstable and toxic sequences in standard plasmids, with high efficiency.
Format
Endura Competent Cells
Clone and maintain unstable or repetitive sequences and lentiviral libraries with high efficiency. Ideal for GeCKO CRISPR library transformation.
Format
TransforMax EC100 Competent Cells
Clone and sub-clone with high-efficiency, recA, endA competent cells.
Size
E. cloni 10G Chemically Competent Cells
Clone, construct libraries, transform Gibson Assembly® reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and challenging applications.
Size
E. cloni EXPRESS Competent Cells
Get better colony counts for routine protein expression with any E. coli T7 expression vector, with this high-efficiency BL21(DE3) strain.
Added to basket
Product information
The HI-Control™ strains are based on E. cloni 10G ( DH10B) and BL21(DE3) strains. These strains harbor a plasmid bearing a constitutive lacI repressor gene, which provides tight control over promoters containing the lacO operator.
| Tightly Control Protein Expression | ||||
| T7 Promoters | Non-T7 lac promoters (T5-lac, tac, trc, lac) |
Clone with low background target expression | Transformation efficiencies | |
| HI-Control BL21(DE3) |
X | X | ≥ 1×107 cfu/µg | |
| HI-Control 10G |
X | X | ≥ 1×109 cfu/µg | |
Genotypes:
HI-Control BL21 (DE3): F- ompT hsdSB (rB- mB-) gal dcm (DE3) Mini-F lacIq1(GentR)
HI-Control 10G: mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 ɸ80dlacZΔM15 ΔlacX74 araD139 Δ (ara,leu)7697 galU galK rpsL (StrR) nupG λ− tonA Mini-F lacIq1 (GentR)
Related Products
Large or Difficult Cloning
TransforMax EPI300-T1R Electrocompetent Cells
Clone large inserts in standard plasmids, avoid T1 and T5 phage contamination, and control copy number of CopyControl vectors.
Phage Display Library Construction
TG1 Electrocompetent Cells
Construct larger, more complex phage display libraries with highest efficiency cells. This amber suppressor strain can be used for phage display and protein expression.
Phage Display Library Construction
MC1061 F-Electrocompetent Cells
High efficiency F-control strain for phage display applications.
Protein Expression
OverExpress Electrocompetent Cells
Express difficult or toxic proteins using standard E. coli T7 expression vectors using these unique strains. Also available in chemically competent format.
Phage Display Library Construction
SS320 (MC1061 F') Electrocompetent Cells
Construct larger, more complex phage display libraries with highest efficiency cells. SS320 is a non-amber suppressor strain used for phage display.
Competent cells for transformation
TransforMax EC100D Electrocompetent E. coli
Perform plasmid rescue for transposition mutants created with the EZ-Tn5 Transposome Kits.
Phage Display Library Construction
ER2738 Electrocompetent Cells
Create large phage display libraries with high efficiency cells recommended for peptide display.
Size
E. cloni 10G Classic Electrocompetent Cells, Efficiency >5 x 109 cfu/µg
Clone, construct libraries, transform Gibson Assembly® reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and challenging applications.
Size
E. cloni 10GF' Electrocompetent Cells, Efficiency >2 x 1010 cfu/µg
Clone, construct libraries, transform Gibson Assembly® reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and challenging applications.
General Cloning and Library Construction
E. cloni 10G Supreme Electrocompetent Cells, Efficiency >4 x 1010 cfu/µg
Clone, construct libraries, transform Gibson Assembly® reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and challenging applications.
General Cloning and Library Construction
E. cloni 10G Elite Electrocompetent Cells, Efficiency >2 x 1010 cfu/µg
Clone, construct libraries, transform Gibson Assembly® reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and challenging applications.
Low Endoxin Cells
ClearColi BL21(DE3) Electrocompetent Cells
Eliminate endotoxin at the source with this unique engineered E. coli strain for low-endotoxin protein expression.
General Cloning and Library Construction
E. cloni 5-alpha Chemically Competent Cells
Clone and subclone with high efficiency, chemically competent DH5-alpha cells.
Added to basket
Please note that the SUMO Protease and SUMO Fusion tag are modified from the original native proteins. Native or non-modified control proteins or proteases will not be compatible.
SDS
Manuals and user guides
You may also be interested in...
Large or Difficult Cloning
CopyControl Induction Solution
Induce CopyControl Fosmid clones to higher-copy number.
Recovery Media
Expression Recovery Medium
Optimised formulation for ensuring stability and high-efficiency transformation of protein expression clones.
Large or Difficult Cloning
CopyCutter Induction Solution
Induce plasmid copy number in CopyCutter EPI400 strain, for high plasmid yields.
Size
TransforMax EPI300
Clone large inserts in standard plasmids, and control copy number of CopyControl vectors. EPI300 Electrocompetent cells are recommended for Gibson Assembly® reactions.
Added to basket
Access support
Need some support with placing an order, setting up an account, or finding the right protocol?
Contact us