Large or Difficult Cloning
E. cloni 5-alpha Chemically Competent Cells
Product Details
ShowCompetent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
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E. cloni 5-alpha Chemically Competent Cells
Clone and subclone with high efficiency, chemically competent DH5-alpha cells.
Key features
Show- Direct replacements for standard cloning strains
- Optimised genetics for high yields: endonuclease and recombination minus, for blue/white screening-capable.
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E. cloni 10G Chemically Competent Cells
Clone, construct libraries, transform Gibson Assembly® reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and challenging applications.
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Product information
E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™ and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is required. These cells provide solutions for a wide range of applications at economical prices.
E. cloni 5-alpha Chemically Competent Cells >1 × 10 8 cfu/µg DNA
Useful for routine cloning, subcloning, and plasmid isolation with or without blue/white screening.
Table 1. E. cloni Competent Cells for Cloning
E. cloni Cell Lines |
Transformation Efficiency |
Cloning Methylated DNA |
BAC, Cosmid Cloning |
Blue/White Screening |
|
5-alpha Chemically Competent |
>1 × 10 8 cfu/µg DNA |
NO |
NO |
YES |
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Genotype
E. cloni 5-alpha: fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17
Efficiencies
E. cloni 5-alpha Chemically Competent Cells >1 × 108 cfu/µg DNA
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