Savings25
CircLigase II ssDNA Ligase
Product Details
ShowCircLigase II ssDNA Ligase is provided at 100 U/μl.
Contents: CircLigase II ssDNA Ligase, CircLigase II 10X Reaction Buffer, 50 mM MnCl2, CircLigase ssDNA Control Oligo, Betaine, Sterile Water.
CircLigase II ssDNA Ligase
Key features
Show- Intramolecular ligation of single-stranded DNA and RNA ends containing 5'-phosphates and 3'-hydroxyl groups
- ATP-independent, non-catalytic enzyme
- Circularises ssDNA 15 bases long
- Compatible with high temperature ligation reactions due to thermostability of CircLigase II enzyme
Product information
Unit Definition: One unit of CircLigase II enzyme converts 1 pmol of a linear 5´-phosphorylated CircLigase II Standard 55-mer Oligo into an exonuclease I-resistant circular form in 1 hour at 60 °C under standard assay conditions.
Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton ® X-100.
10X Reaction Buffer: 0.33 M Tris-Acetate (pH 7.5), 0.66 M potassium acetate and 5 mm DTT.
Quality Control: CircLigase II ssDNA Ligase is free of detectable phosphatase, DNA exo- and endonuclease, and RNase activities.
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