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MC1061 F-Electrocompetent Cells

MC1061 F-Electrocompetent Cells

Product Details

Competent Cells include Control DNA and Recovery Medium and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

MC1061 F-Electrocompetent Cells

High efficiency F-control strain for phage display applications.

Key features

Perfect for antibody phage display or peptide phage display library creation
The highest efficiency TG1 competent cells available
Your only source for electrocompetent SS320 and ER2738 cells
Amber suppressor and non-amber suppressor lines for maximum flexibility
High efficiency MC1061 F- cells for cloning or control reactions
Create larger libraries; speed discovery
Bulk custom dispensing available with all of our phage display cells

Select a Size

24 reactions (DUOs)

12 reactions (DUOs)

TBD

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Product information

MC1061 F- Electrocompetent Cells. A non-amber suppressor strain, identical to SS320 except that it lacks the F′episome. Highly efficient (≥4 × 10¹⁰ cfu/µg) electrocompetent cells for general cloning or Phage Display. Note that as an F- strain, it cannot be used for re-infection by filamentous phage. This strain not included in the sample kit.

Transformation efficiency of Biosearch Technologies electrocompetent bacterial cells for phage display compared to competitors

Figure 1. Transformation efficiency of electrocompetent bacterial cells for phage display compared to competitor's specification.

Table 1. Competent Cells for Phage Display libraries

Cell Lines	Transformation Efficiency (cfu/µg pUC DNA)	Cloning Methylated DNA	BAC, Cosmid Cloning	Blue/White Screening
TG1 Electrocompetent	>4 × 10¹⁰	YES	NO	YES IPTG induction required
SS320 (MC1061 F') Electrocompetent	>4× 10¹⁰	YES	NO	YES IPTG induction required
MC1061 F- Electrocompetent	>4× 10¹⁰	YES	NO	YES IPTG induction required
ER2738 Electrocompetent	>2 × 10¹⁰	YES	NO	YES IPTG induction required

Genotype

TG1: [F' traD36 proAB lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK - mK -)

SS320 (MC1061F'): F'[proAB lacI^qZ ΔM15 Tn10 (Tet^R)] araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (Str^R) hsdR2 (r_K– m_K+) mcrA mcrB1

ER2738: [F'proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10 (tetr)] fhuA2 glnVΔ(lac-proAB) thi-1Δ(hsdS-mcrB)5

MC1061 F-: F-, araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (Str^R) hsdR2 (r_K– m_K+) mcrA mcrB1

SDS

Manuals and user guides

Product information sheets

F302 US phage display comp cells

Application notes

technical note E.coli strains for phage display

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