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TransforMax EC100 Competent Cells

TransforMax EC100 Competent Cells

Product Details

Includes pUC19 control DNA.

TransforMax EC100 Competent Cells

Clone and sub-clone with high-efficiency, recA, endA competent cells.

Key features

General cloning strain
Clone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA.
Achieve high efficiencies with convenient chemically competent format.
Ideal for cloning, subcloning and plasmid preparation due to endA and rec mutations.

Option 1: Select a Format

electrocompetent

chemically competent

Option 2: Select a Size

20 reactions

10 reactions

TBD

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Product information

The highly versatile TransforMax™ EC100™ E. coli competent cells are ideal for most cloning applications. The cells provide very high transformation efficiency when tested against a wide range of supercoiled DNAs as well as DNA directly from a ligation reaction (Table. 1).

Applications

Routine cloning of DNA up to 200 kb.

Benefits

High transformation efficiency with clones of all sizes, including BAC clones (Table. 1).
lacZΔM15 for blue/white screening of recombinants.
Restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] enables efficient cloning of methylated DNA.
Endonuclease minus (endA1) to ensure high yields of DNA.
Recombination minus (recA1) for greater stability of large cloned inserts.

DNA	TransforMax EC100 Chemically Competent E. coli	TransforMax EC100 Electrocompetent E. coli
pUC19	1.4 × 10⁸	1.4 × 10¹⁰
8.1-kb Clone	1.3 × 10⁷	Not tested
13.1-kb Clone	4.3 × 10⁶	1.3 × 10⁹
23.1-kb Clone	9.2 × 10⁵	3.0 × 10⁸
145-kb BAC Clone	Not tested	7 × 10⁷
13.1-kb clone directly from a ligation reaction	2.2 × 10⁵	2.1 × 10⁷

Table. 1. Comparison of the transformation efficiencies of TransforMax™ EC100™ E. coli with a variety of DNAs. Transformations were performed using 50 µL of competent cells and either supercoiled DNAs of the indicated sizes or a 1-µL aliquot from a standard 10-µL ligation reaction. Results shown are in cfu/µg of DNA and are the average transformation efficiencies obtained from several trials.

Genotype

F^- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ^- rpsL (Str^R) nupG

TransforMax EC100 Electrocompetent E. coli

Transformation efficiency of >1 × 10¹⁰cfu/µg of pUC19.

TransforMax EC100 Chemically Competent E. coli

Transformation efficiency of >5 × 10⁸cfu/µg of pUC19.

SDS

CC02810 TransforMax EC100 Chemically Competent Ecoli

Manuals and user guides

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