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Photocleavable Modifications

Explore our photocleavable analogues of the Amino- and Spacer-Modifiers and the Biotin label, as well as our PC linker molecule. The general design of the PC monomers is based on an α-substituted 2-nitrobenzyl group. The photo-reactive group is derivatised as a cyanoethyl phosphoramidite that can be incorporated during automated DNA synthesis.

Photocleavable Modifications

Photocleavable Modifications Modification Application
PC 5'-Amino-Modifier CE-Phosphoramidite Terminal modification that results in a 5'-monophosphate on the released oligonucleotide.
  • Affinity conjugation and purification
  • Compatible with post-synthetic modification with amine reactive reagents
PC 5'-Biotin CE-Phosphoramidite Terminal modification that results in a 5'-monophosphate on the released oligonucleotide. This phosphoramidite contains a biotinyl moiety that bears a trityl group on the N-1 nitrogen atom for N-protection.
  • Oligonucleotide isolation and purification
  • Compatible with streptavidin methods
PC Linker CE-Phosphoramidite Mid-sequence modification that results in monophosphate fragments at both the 3'- and 5'-termini.
  • Mass markers
  • Photo-triggered strand cleavage applications
PC Spacer CE-Phosphoramidite Mid-sequence modification that results in a 5'-monophosphate on the released oligonucleotide.
  • Mass markers

Photocleavage using PC Linker Phosphoramidite

Photo-triggered DNA cleavage is an important tool used for studying conformational changes and strand breaks, as well as for studying activation of nucleic-acid-targeted drugs, such as antisense oligonucleotides.


DNA Purification using PC-5’-Biotin Phosphoramidite

This technique is commonly used to isolate DNA by first hybridisation to the biotin labelled probe, then release of the probe/DNA duplex after photolysis. After photo-cleavage the DNA is suitable for further biological manipulations like gene construction and cloning after ligation.