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DNA Polymerase I Large (Klenow) Fragment

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DNA Polymerase I Large (Klenow) Fragment includes Klenow DNA Polymerase at 5 U/µL and 10X DNA Polymerase Buffer A

DNA Polymerase I Large (Klenow) Fragment

Klenow is the large fragment of DNA polymerase I that retains the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities of intact DNA Polymerase I but lacks the 5' to 3' exonuclease activity. It is provided at 5 U/µL.

Key features

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  • Forms blunt ends by removing 3’ overhangs or filling in 5’ overhangs
  • Used in second-strand cDNA synthesis
  • Generates DNA probes using random primers
Item ID 30098-1
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Product information

DNA Polymerase I, Large (Klenow) Fragment is a DNA-dependent polymerase that lacks the 5′→3′ exonuclease activities of E. coli DNA Polymerase I from which it is derived. The 3′→5′ remains intact to remove 3’ overhangs, and along with the polymerase activity to fill in 5’ overhangs and create blunt end DNA fragments.  The optimal incubation temperature of the enzyme is 25 °C and uses 10X DNA Polymerase Buffer A. Heat inactivation of the enzyme is 75 °C for 20 minutes. 

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