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BAC-Optimised Electrocompetent Cells

BAC-Optimised Electrocompetent Cells

Product Details

Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube) or FIVERs (5 transformations per tube), as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

BAC-Optimised Electrocompetent Cells

Key features

Cells that enable the most challenging library construction and screening.
Highest available transformation efficiencies for library construction and screening.
Optimised for high yields of DNA.

Select a Size

12 reactions (DUOs)

24 reactions (DUOs)

TBD

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Product information

We offer two bacterial strains designed for BAC library construction or transformation of large constructs:

BAC-Optimised Replicator™ v2.0 Electrocompetent Cells (> 1 × 10¹⁰ cfu/µg pKanR DNA). These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields. BAC-Optimised Replicator Cells are components of Biosearch Technologies Cloning Kits

E. cloni 10G BAC-Optimised Electrocompetent Cells (> 1 × 10⁷ cfu/µg 150 kb BAC DNA; > 1 × 10¹⁰ cfu/µg pUC DNA). The first competent cells developed specifically for large DNA cloning. Manufactured using a novel procedure that maximises transformation efficiencies with very large inserts, 10G BAC-Optimised Cells are an excellent choice for BAC library construction and large construct transformation.

Table 1. E. cloni Competent Cells for BAC or Large Fragment Cloning

*E. cloni* Cell Lines	Transformation Efficiency (cfu/µg pUC or pKanR DNA)	Cloning Methylated DNA	BAC, Cosmid Cloning	Blue/White Screening
10G BAC-Optimised Electrocompetent	>1 × 10¹⁰	YES	YES	YES without IPTG induction
BAC-Optimised Replicator v2.0 Electrocompetent	>1 × 10¹⁰	YES	YES	YES IPTG induction required

Replicator v2.0: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galUgalK rpsL nupG (attL araC-PBAD-trfA250 bla attR) λ-. Note that Replicator v2.0 cells are AmpR (bla gene) (StrR)

E. cloni 10G BAC-Optimized: F - mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupG λ- tonA (StrR)

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