Protein Expression
HI-Control BL21(DE3) Chemically Competent Cells
Induce high-level protein expression from T7 promoters with tight control over leaky expression, using this strain containing a LacI repressor.
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Endura Competent Cells
Product Details
ShowEndura™ Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube) or DUOs (2 transformations per tube) as indicated. Endura Electrocompetent cells are provided in DUOs format only. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
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Endura Competent Cells
Clone and maintain unstable or repetitive sequences and lentiviral libraries with high efficiency. Ideal for GeCKO CRISPR library transformation.
Key features
Show- Clone repetitive sequences and lentiviral libraries
- Stabilise direct repeats and create lentiviral constructs
- Generate CRISPR-GeCKO-Libraries-Endura lentiviral guide RNA libraries
- Recommended in CRISPR GeCKO library protocols
- Choose electrocompetent or chemically competent cells
- Highest efficiency commercially available cells for lentiviral cloning
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Get better colony counts for routine protein expression with any E. coli T7 expression vector, with this high-efficiency BL21(DE3) strain.
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Product information
Maintain unstable DNA.
Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be propagated stably.Excellent value and efficiency! Switch to Endura cells today and experience a higher level of efficiency and a savings in your cloning budget.
Chemical or Electrocompetent. Whichever method of transformation you prefer, we can give you better efficiency and/or better prices.
Figure 2. Transformation efficiencies of electrocompetent clone-stabilising strains.
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TransforMax EPI300-T1R Electrocompetent Cells
Clone large inserts in standard plasmids, avoid T1 and T5 phage contamination, and control copy number of CopyControl vectors.
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Construct larger, more complex phage display libraries with highest efficiency cells. This amber suppressor strain can be used for phage display and protein expression.
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High efficiency F-control strain for phage display applications.
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Express difficult or toxic proteins using standard E. coli T7 expression vectors using these unique strains. Also available in chemically competent format.
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Construct larger, more complex phage display libraries with highest efficiency cells. SS320 is a non-amber suppressor strain used for phage display.
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Perform plasmid rescue for transposition mutants created with the EZ-Tn5 Transposome Kits.
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Create large phage display libraries with high efficiency cells recommended for peptide display.
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E. cloni 10G Supreme Electrocompetent Cells, Efficiency >4 x 1010 cfu/µg
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ClearColi BL21(DE3) Electrocompetent Cells
Eliminate endotoxin at the source with this unique engineered E. coli strain for low-endotoxin protein expression.
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Genotype:
recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)
SDS
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60240 Endura Chemically Competent Cells Duo
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60241 Endura Chemically Competent Cells Solo
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60242 Endura ElectroCompetent Cells
Manuals and user guides
Product information sheets
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