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  4. E. cloni 10G Chemically Competent Cells

E. cloni 10G Chemically Competent Cells

E. cloni 10G Chemically Competent Cells

Product Details

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Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube), DUOs (2 transformations per tube), Subcloning Grade (12 transformations per tube), or microplates as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

E. cloni 10G Chemically Competent Cells

Clone, construct libraries, transform Gibson Assembly® reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and challenging applications.

Key features

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  • Direct replacements for standard cloning strains
  • Optimised genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable.
  • Available in a range of high transformation efficiencies
  • Convenient packaging options.
Option 1: Select a Size
Option 2: Select a Efficiency
TBD
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Product information

E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α&trade, DH10B&trade, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability (Figures. 1 and 2), while decreasing costs. These cells provide solutions for a wide range of applications at economical prices.

Choice of strain:

  • E. cloni 10G Competent Cells. Library construction, cloning, subcloning, and plasmid isolation with or without blue/white screening.
  • E. cloni 10GF′ Competent Cells. Contain the F' plasmid for infection with M13 to produce ssDNA.

Choice of efficiency:

  • E. cloni 10G SUPREME Electrocompetent Cells > 4 × 1010 cfu/µg pUC DNA
    SUPREME Cells have the highest transformation efficiency available from any supplier. Choose SUPREME Cells for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.
  • E. cloni 10G & 10GF´ ELITE Electrocompetent Cells > 2 × 1010 cfu/µg pUC DNA
    ELITE Cells have twice the transformation efficiency compared to “ultra high efficiency” cells from other suppliers. ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price.
  • E. cloni 10G CLASSIC Electrocompetent Cells > 5 × 109 cfu/µg pUC DNA
    CLASSIC Cells are high efficiency cells with a substantially lower cost per reaction. These cells are the most economical choice for standard cloning and library construction. 10G CLASSIC Cells are available in larger package sizes for convenient use in higher volume cloning applications.

 

Custom Competent Cells

 

Bulk cells or your strain of E. coli, made to the highest possible transfection efficiency with custom dispensing! See Custom Competent Cells page for detailed information.

Plasmid cloning. E. cloni 10G SUPREME Cells provide recombinant yields higher than the highest efficiency cells offered by a leading supplier (Figures 1 and 2)


Recombinant Yields

 

Transformation Efficiency

Figure 1. E. cloni 10G SUPREME Electrocompetent Cells consistently outperformed “ultra high efficiency” cells from a leading supplier. Both strains were transformed with 10 pg of pUC19 (n=16).

 

Figure 2.Transformation efficiency comparison of E. cloni 10G SUPREME Cells and Mega DH10B cells (Invitrogen). Competent cells were transformed according to the manufacturer’s instructions with the same pUC19 DNA control (provided with the Invitrogen cells)

Table 1. E. cloni Competent Cells for Cloning and Library Construction


E. cloni Cell Lines

Transformation Efficiency
(cfu/µg pUC DNA)

Cloning Methylated DNA

BAC, Cosmid Cloning

Blue/White Screening

10G SUPREME Electrocompetent

>4 × 1010

YES

YES

YES
without IPTG induction

10G ELITE Electrocompetent

>2 × 1010

YES

NO

YES
without IPTG induction

10G CLASSIC Electrocompetent

>5 × 109

YES

NO

YES
without IPTG induction

10GF´ ELITE Electrocompetent

>2 × 1010

YES

NO

YES
IPTG induction required

Genotypes

E. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697galU galK rpsL nupG λ- tonA (StrR)

E. cloni 10GF´: [F´ pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupG λ- tonA (StrR)

Efficiencies

E. cloni 10G SUPREME Electrocompetent Cells > 4 × 1010 cfu/µg pUC DNA

E. cloni 10G & 10GF´ ELITE Electrocompetent Cells > 2 × 1010 cfu/µg pUC DNA

E. cloni 10G CLASSIC Electrocompetent Cells > 5 × 109 cfu/µg pUC DNA 

SDS

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