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  3. Unmodified Oligo Synthesis Reagents
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  6. 5'-DMT-T-3'-Q Linker CPG Column

5'-DMT-T-3'-Q Linker CPG Column

5'-DMT-T-3'-Q Linker CPG Column
5'-DMT-T-3'-Q Linker CPG Column
5'-DMT-T-3'-Q Linker CPG Column
5'-DMT-T-3'-Q Linker CPG Column
5'-DMT-T-3'-Q Linker CPG Column
5'-DMT-T-3'-Q Linker CPG Column
5'-DMT-T-3'-Q Linker CPG Column
5'-DMT-T-3'-Q Linker CPG Column

5'-DMT-T-3'-Q Linker CPG Column

CPG synthesis column for incorporation of unmodified dT at 3' end of an oligonucleotide.

Key features

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  • CPG has Q-linker for quick cleavage (2 minutes at room temperature)
  • Ideal for base sensitive or dye labelled oligos
Option 1: Select a Pore Size
Option 2: Select a Column Type
Option 3: Select a Scale
TBD
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Product information

Various DNA CPG column formats are available, and compatible with a range of instruments. 

Synthesizer
Column
Type/Description
Notes
MerMade 6,12
MerMade, syringe (all scales)
Pipette type column
A MerMade column is also known as a Supercolumn
MerMade 4, 48X, 96E, 192E, 192X
MerMade, Syringe (up to 1.3 mL)
Pipette type column
A MerMade column is also known as a Supercolumn
ABI 384 / 394
Luer
Barrel column with luer fitting at either end
Also known as ALL-FIT or Standard
Expedite 8909
Luer
Barrel column with luer fitting at either end
Also known as ALL-FIT or Standard
ABI3900
MerMade
Pipette type column
A MerMade column is also known as a Supercolumn
K&A H4, H8, H8SE, H2, H32, H64
Luer
Barrel column with luer fitting at either end
Also known as ALL-FIT or Standard
K&A S4CL/S8CL
Luer
  Barrel column with luer fitting at either end 
 Also known as Standard. For this instrument, we recommend the Luer (Standard) column as the ALL-FIT columns have a wider barrel.
Dr Oligo 48
MerMade
Pipette type column
A MerMade column is also known as a Supercolumn
 Dr Oligo 192XLc, 768XLc just plates 
 MerMade, Syringe (up to 1.3 mL) 
Pipette type column
A MerMade column is also known as a Supercolumn
 OligoMaker X12, 48, 192, X192, X96 
MerMade, Syringe (up to 1.3 mL)
Pipette type column
A MerMade column is also known as a Supercolumn

Properties:

  • Appearance: White Powder
  • Extinction Coefficient at 260 nm: 8400

Spectral properties measured in water, for the cleaved and deprotected nucleoside.

Product usage:

  • Cleavage conditions: Cleavage can be carried out in 2 minutes at room temperature using concentrated NH4OH.
  • Deprotection conditions: If fast deprotecting amidites are used, deprotection can be carried out using concentrated NH4OH for 1 hour @ 60 °C.
    • Image of cleaved and deprotected structure:
  • The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 304.19

Storage and handling:

  • Shipping conditions: Ambient
  • Storage conditions: +2 to +8 °C in sealed container

SDS

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