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  6. 5'-DMT-T-3'-Q Linker CPG Column

5'-DMT-T-3'-Q Linker CPG Column

5'-DMT-T-3'-Q Linker CPG Column

5'-DMT-T-3'-Q Linker CPG Column

CPG synthesis column for incorporation of unmodified dT at 3' end of an oligonucleotide.

Key features

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  • CPG has Q-linker for quick cleavage (2 minutes at room temperature)
  • Ideal for base sensitive or dye labelled oligos
Option 1: Select a Pore Size
Option 2: Select a Column Type
Option 3: Select a Scale
TBD
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Product information

Various DNA CPG column formats are available, and compatible with a range of instruments.

Column Type Description Compatible synthesizers
Hybrid columns
  • Pipette format
  • Medium porosity Porex frits
  • Leaves headspace above the CPG
  • MerMade
  • ABI 3900
  • Other pipette-based synthesizers
Supercolumns or MerMade columns
  • Upper pipette fitting and a lower luer fitting
  • Frit stomped to remove headspace over CPG
  • MerMade
  • ABI 3900
  • Dr Oligo
  • Other pipette-based synthesizers
Standard or ALL-FIT columns
  • Luer-to-luer fitting
  • ABI 394
  • Expedite 8900/8909
  • Other luer-to-luer fitting synthesizers such as K&A
TWIST™ columns
  • Luer-to-luer fitting
  • Have a screw cap that can be opened to manipulate the synthesis support and then resealed
  • Generally used for larger scale synthesis
  • Luer-to-luer fitting synthesizers

Properties:

  • Appearance: White Powder
  • Extinction Coefficient at 260 nm: 8400

Spectral properties measured in water, for the cleaved and deprotected nucleoside.

Product usage:

  • Cleavage conditions: Cleavage can be carried out in 2 minutes at room temperature using concentrated NH4OH.
  • Deprotection conditions: If fast deprotecting amidites are used, deprotection can be carried out using concentrated NH4OH for 1 hour @ 60 °C.
    • Image of cleaved and deprotected structure:
  • The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 304.19

Storage and handling:

  • Shipping conditions: Ambient
  • Storage conditions: +2 to +8 °C in sealed container

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