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SS320 (MC1061 F') Electrocompetent Cells

SS320 (MC1061 F') Electrocompetent Cells

Product Details

Competent Cells include Control DNA and Recovery Medium and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

SS320 (MC1061 F') Electrocompetent Cells

Construct larger, more complex phage display libraries with highest efficiency cells. SS320 is a non-amber suppressor strain used for phage display.

Key features

Perfect for antibody phage display library creation
Create larger libraries; speed discovery
Bulk custom dispensing available with all of our phage display cells

Select a Size

12 reactions (DUOs)

24 reactions (DUOs)

TBD

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Product information

SS320 Electrocompetent Cells. A non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (≥4 × 10¹⁰ cfu/µg) electrocompetent cells for phage display library screening. These cells have the highest available transformation efficiency of any strain used for phage display.

Transformation efficiency of Biosearch Technologies electrocompetent bacterial cells for phage display compared to competitors

Figure 1. Transformation efficiency of electrocompetent bacterial cells for phage display compared to competitor's specification.

Table 1. Competent Cells for Phage Display libraries

Cell Lines	Transformation Efficiency (cfu/µg pUC DNA)	Cloning Methylated DNA	BAC, Cosmid Cloning	Blue/White Screening
TG1 Electrocompetent	>4 × 10¹⁰	YES	NO	YES IPTG induction required
SS320 (MC1061 F') Electrocompetent	>4× 10¹⁰	YES	NO	YES IPTG induction required
MC1061 F-Electrocompetent	>4× 10¹⁰	YES	NO	YES IPTG induction required
ER2738 Electrocompetent	>2 × 10¹⁰	YES	NO	YES IPTG induction required

Genotype

TG1: [F' traD36 proAB lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK - mK -)

SS320 (MC1061F'): F'[proAB lacI^qZ ΔM15 Tn10 (Tet^R)] araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (Str^R) hsdR2 (r_K– m_K+) mcrA mcrB1

ER2738: [F'proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10 (tetr)] fhuA2 glnVΔ(lac-proAB) thi-1Δ(hsdS-mcrB)5

MC1061 F-: F-, araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (Str^R) hsdR2 (r_K– m_K+) mcrA mcrB1

SDS

Manuals and user guides

Product information sheets

F302 US phage display comp cells

Application notes

technical note E.coli strains for phage display

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