Large or Difficult Cloning
TransforMax EC100D Electrocompetent E. coli
Product Details
ShowEach product contains 5 tubes each containing 100 µL of electrocompetent cells, which is enough cells for 10 electroporations. A control vector containing and R6Kγori is also included with each product.
You are currently seeing list prices, to see your prices please log in
TransforMax EC100D Electrocompetent E. coli
Perform plasmid rescue for transposition mutants created with the EZ-Tn5 Transposome Kits.
Key features
Show- Rescue cloning of EZ-Tn5 transposed elements
- Propagate plasmids tagged with the EZ-Tn5 Insertion Kit
Added to basket
Similar Products
Large or Difficult Cloning
TransforMax EPI300-T1R Electrocompetent Cells
Clone large inserts in standard plasmids, avoid T1 and T5 phage contamination, and control copy number of CopyControl vectors.
Phage Display Library Construction
TG1 Electrocompetent Cells
Construct larger, more complex phage display libraries with highest efficiency cells. This amber suppressor strain can be used for phage display and protein expression.
Phage Display Library Construction
MC1061 F-Electrocompetent Cells
High efficiency F-control strain for phage display applications.
Protein Expression
OverExpress Electrocompetent Cells
Express difficult or toxic proteins using standard E. coli T7 expression vectors using these unique strains. Also available in chemically competent format.
Phage Display Library Construction
SS320 (MC1061 F') Electrocompetent Cells
Construct larger, more complex phage display libraries with highest efficiency cells. SS320 is a non-amber suppressor strain used for phage display.
Low Endoxin Cells
ClearColi BL21(DE3) Electrocompetent Cells
Eliminate endotoxin at the source with this unique engineered E. coli strain for low-endotoxin protein expression.
Phage Display Library Construction
ER2738 Electrocompetent Cells
Create large phage display libraries with high efficiency cells recommended for peptide display.
Added to basket
Product information
The TransforMax™ EC100D™ pir + Electrocompetent E. coli and TransforMax™ EC100D™ pir-116 Electrocompetent E. coli each constitutively express the π protein (the pir gene product) for replication of plasmids containing the R6Kγ origin of replication (R6Kγ ori). The cells are derived from Epicentre's TransforMax™ EC100™ Electrocompetent E. coli by P1 phage transduction with a strain containing the pir + or pir-116 gene linked to a dihydrofolate reductase (DHFR) marker.
The TransforMax EC100D pir + cells will maintain R6Kγ ori containing plasmids at approximately 15 copies per cell, 1 for cloning of potentially toxic or unstable DNA sequences. The TransforMax EC100D pir-116 cells are for high-copy propagation of up to 250 rescue plasmid copies per cell. 1
Benefits
- Accepts large clones for unbiased propagation and stability of large rescue clones.
- lacZΔM15 for blue/white screening of recombinants.
- Restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] enables efficient cloning of methylated DNA.
- Endonuclease minus ( endA1) to ensure high yields of DNA.
- Recombination minus ( recA1) for greater stability of large cloned inserts.
Genotypes
TransforMax EC100D pir
+ Electrocompetent E. coli:
F - mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (Str R) nupG pir +(DHFR)
TransforMax EC100D pir-116 Electrocompetent E. coli:
F - mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (Str R) nupG pir-116(DHFR)
Transformation Efficiency
Greater than 5 × 10 9 colony forming units (cfu)/µg with pR6Kan control DNA.
References
- Metcalf, W.W. et al. (1994) Gene 138, 1.
Related Products
General Cloning and Library Construction
HI-Control 10G Chemically Competent Cells
Stabilise toxic sequences behind T7 or lac-based promoters with this strain containing a LacI repressor, providing tight control over leaky expression.
Protein Expression
HI-Control BL21(DE3) Chemically Competent Cells
Induce high-level protein expression from T7 promoters with tight control over leaky expression, using this strain containing a LacI repressor.
Protein Expression
OverExpress Chemically Competent Cells
Express difficult or toxic proteins using standard E. coli T7 expression vectors using these unique strains. Some strains also available in electrocompetent format.
General Cloning and Library Construction
E. cloni 5-alpha Chemically Competent Cells
Clone and subclone with high efficiency, chemically competent DH5-alpha cells.
Large or Difficult Cloning
CopyCutter EPI400 Competent Cells
Clone unstable and toxic sequences in standard plasmids, with high efficiency.
Format
Endura Competent Cells
Clone and maintain unstable or repetitive sequences and lentiviral libraries with high efficiency. Ideal for GeCKO CRISPR library transformation.
Format
TransforMax EC100 Competent Cells
Clone and sub-clone with high-efficiency, recA, endA competent cells.
Size
E. cloni 10G Chemically Competent Cells
Clone, construct libraries, transform Gibson Assembly® reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and challenging applications.
Size
E. cloni EXPRESS Competent Cells
Get better colony counts for routine protein expression with any E. coli T7 expression vector, with this high-efficiency BL21(DE3) strain.
Added to basket
You may also be interested in...
Large or Difficult Cloning
CopyControl Induction Solution
Induce CopyControl Fosmid clones to higher-copy number.
Recovery Media
Expression Recovery Medium
Optimised formulation for ensuring stability and high-efficiency transformation of protein expression clones.
Large or Difficult Cloning
CopyCutter Induction Solution
Induce plasmid copy number in CopyCutter EPI400 strain, for high plasmid yields.
Size
TransforMax EPI300
Clone large inserts in standard plasmids, and control copy number of CopyControl vectors. EPI300 Electrocompetent cells are recommended for Gibson Assembly® reactions.
Added to basket
Access support
Need some support with placing an order, setting up an account, or finding the right protocol?
Contact us