The sbeadex Pathogen Nucleic Acid Purification Kit without dangerous goods is a cutting-edge solution for fast and reliable isolation of highly purified DNA and RNA from various pathogenic bacteria, viruses and yeasts.

The sbeadex Technology

This kit leverages the power of sbeadex magnetic bead technology. These superparamagnetic particles, coated with sbeadex surface chemistry, employ a novel two-bind / two-wash mechanism that ensures effective removal of impurities from the sample matrix. The second wash buffer in the kit is ethanol-free, eliminating any traces of ethanol that could potentially inhibit downstream reactions. Additionally, the sbeadex technology eliminates the need for a drying step before elution, further enhancing DNA quality.

The inclusion of Proteinase K facilitates efficient cell disruption and nucleic acid release with certain sample material and safeguards its integrity. The Proteinase K enzyme enhances the efficiency of nucleic acid extraction by digestion of proteins in the cell lysate. This addition helps produce higher DNA/RNA yields and improves the stability of extracted nucleic acids.

Figure 1:  Demonstration of no loss of RNA during the sbeadex Pathogen Nucleic Acid Purification Kit protocol.  500 copies of a 2019-COVID artificial RNA reference material were added to a sample (nasopharyngeal swab) and the sample processed with the sbeadex Pathogen Nucleic Acid Purification Kit. SARS-CoV-2 was detected in this preparation and a directly spiked positive control sample via a RT-qPCR at two separate Biosearch Technologies sites (reaction components described below). The concordant Cq values from both samples demonstrate no loss of sample due to the extraction step.

This is a ready-to-use kit designed to streamline the DNA and RNA purification process, making it an ideal choice for downstream applications such as:

• PCR / qPCR

• Real Time (RT) PCR / qRT-PCR

• Next Generation Sequencing (NGS)

The kit is compatible with a wide range of automation platforms, such as Thermo's KingFisher and Hamilton's Microlab Star or our oKtopure, and can be easily automated on various liquid handlers and purification platforms. It offers high flexibility in reaction volumes, making it suitable for high-throughput purifications.

This kit contains no dangerous goods ensuring an extra level of safety and regulatory compliance.

The sbeadex Pathogen testing kits have been used successfully with:

Table 1: Pathogens that have already been tested using the sbeadex Pathogen Nucleic Acid Purification Kit.

Standards used for testing

1. AccuPlex™ SARS-CoV-2 Verification Panel - Full Genome, 0505-0168 (LGC SeraCare)
2. AMPLIRUN® TOTAL RESPIRATORY VIRAL PANEL CONTROL (SWAB), MBTC020 (Vircell S.L.)
3. AMPLIRUN TOTAL ZIKV/DENV/CHIKV CONTROL (PLASMA), MBTC023 (Vircell S.L.)
4. AMPLIRUN TOTAL CMV CONTROL (PLASMA), MBTC018-R (Vircell S.L.)
5. AccuTrak™ HPV Genotype Qualification Panel, QSH701 (2400-0161), (LGC SeraCare)
6. AMPLIRUN TOTAL ATYPICAL BACTERIAL PNEUMONIA CONTROL (SWAB) MBTC022-R (Vircell S.L.)
7. AMPLIRUN TOTAL MTB CONTROL (SPUTUM) MBTC013 (Vircell S.L.)
8. AMPLIRUN TOTAL GASTROINTESTINAL BACTERIAL PANEL CONTROL (STOOL), MBTC021 (Vircell S.L.)
9. AMPLIRUN TOTAL CT/NG CONTROL (URINE), MBTC003 (Vircell S.L.)
10. NATtrol™ S. aureus (MRSA) External Run Control, NATSAU(MRSA)-ERCM (Zeptometrix Corporation)
11. NATtrol Candida/TV Positive Control, NATCTVPOS-BD (Zeptometrix Corporation)

Note: Other sample matrices may also be used with this kit. Further testing on other pathogens and matrices is in progress and this table will be updated accordingly.