The MasterPure™ DNA Purification Kit for Blood Version II can be used to isolate exceptionally pure genomic DNA from whole blood or buffy coat. With the optimised protocol for DNA, up to 100 µg of DNA can be recovered from 600 µl of buffy coat in less than 30 minutes (Table 1). The purified DNA contains both high- and low-molecular-weight genomic DNA which can be used directly as a template in PCR amplification reactions (Figure. 1), for digestion with restriction enzymes (Figure. 2), for Southern blotting, or for other molecular biology applications. Protocols are provided for genomic DNA and TNA purification from whole blood and buffy coat. For RNA samples, we recommend the MasterPure RNA Purification Kits. Both protocols allow for scaling up to accommodate larger samples.