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Does the RapiDxFire qPCR 5X Master Mix GF contain a passive reference dye?

PCRAMP-012

No, the RapiDxFire qPCR 5X Master Mix GF does not contain a passive reference dye. However, customers can supplement their reaction mix with a passive reference dye of their choice.

Is BHQ Probe Master Mix stable at room temperature?

BHQ-001

BHQ Probe Master Mix is an incredibly stable PCR/qPCR Master Mix, which shows little-to-no reduction in performance when left at room temperature, even when combined with all other PCR-reaction components. We have demonstrated Cq > 1.0 when assembled qPCR reactions were left at room temperature for 16 hours, in both singleplex and multiplex applications.

To maintain high performance, it is recommended to store BHQ Probe Master Mix at -20 °C, and aliquot it into nuclease-free, light-protected tubes to minimise the number of freeze-thaw cycles. BHQ Probe Master Mix is also stable at +4 °C for 1 week.

Can I use other dyes besides the Green Fluorescent Dye available in the LavaLAMP™ DNA Component Kit with Dye?

PCRAMP-009, PCRAMP-010, PCRAMP-007, PCRAMP-006

Yes. Syto-13 (Molecular Probes) and EvaGreen (Biotium) have both been tested with the LavaLAMP DNA Master Mix and they perform well.

What is the difference between RapiDxFire™ qPCR 5X Master Mix GF and BHQ™ Probe Master Mix?

PCRAMP-012

RapiDxFire qPCR 5X Master Mix GF is a 5X qPCR master mix produced and purified in a quality standard ISO 13485-certified manufacturing facility, allowing for its use in molecular diagnostic and clinical applications.

BHQ Probe Master Mix is a 2X PCR master mix, which has been validated for both endpoint genotyping and qPCR applications. It has been designed specifically to be inhibitor-tolerant, especially when working with crude-extracted agrigenomic samples (e.g. leaves, seeds).

Can I use other dyes besides the Green Fluorescent Dye available in the LavaLAMP™ DNA Master Mix with Dye?

PCRAMP-009, PCRAMP-010, PCRAMP-007, PCRAMP-006

Yes. Syto-13 (Molecular Probes) and EvaGreen (Biotium) have both been tested with the LavaLAMP DNA Master Mix and they perform well.

What are the storage conditions for BHQ Probe Master Mix?

BHQ-001

BHQ Probe Master Mix should be stored at -20 °C. Repeated freeze-thaws should be avoided, so if BHQ Probe Master Mix is to be aliquoted, nuclease-free, light-protected tubes/vials should be used.

Once thawed, BHQ Probe Master Mix can be stored at +4 °C for 1 week.

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