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Does the RapiDxFire qPCR 5X Master Mix GF contain a passive reference dye?

PCRAMP-012

No, the RapiDxFire qPCR 5X Master Mix GF does not contain a passive reference dye. However, customers can supplement their reaction mix with a passive reference dye of their choice.

Can I use other dyes besides the Green Fluorescent Dye available in the LavaLAMP™ DNA Master Mix with Dye?

PCRAMP-009, PCRAMP-010, PCRAMP-007, PCRAMP-006

Yes. Syto-13 (Molecular Probes) and EvaGreen (Biotium) have both been tested with the LavaLAMP DNA Master Mix and they perform well.

Why did you add dyes to the 500 Series and 700 Series Primers in the NxSeq HT Dual Indexing Kit?

NXGSEQ-003, NXGSEQ-004, 15300-1, NXGSEQ-001, NXGSEQ-002

The goal was to help you ensure that each primer is correctly dispensed into your PCR amplification plate. By making each set of primers a different colour, you can verify that each one was added to every well of an entire row or column during PCR plate setup. We first recommend aliquoting 2.5 µL of each 700 Series Primer into each row of the plate using a 12-channel pipette. Each row should turn blue indicating that the primers were successfully dispensed. Second, we recommend aliquoting 2.5 µL of each 500 Series Primer (orange/yellow colour) into each column of the plate using an 8-channel pipette. As the 500 Series Primers are dispensed, each column on the plate should turn green indicating the successful addition of the 500 Series Primers (blue + orange/yellow = green).

Won’t the dyes in the primers affect my Illumina sequencing run and/or data quality?

NXGSEQ-003, NXGSEQ-004, 15300-1, NXGSEQ-001, NXGSEQ-002

No. We’ve done extensive testing to demonstrate that the dyes do not affect the sequencing run/instruments or decrease the quality of the data generated. During the final library clean-up and size selection steps, the dyes are removed and therefore, do not make it on to the sequencer. To ensure compatibility in case of incomplete dye removal, we added the full concentration of dye found in the PCR amplification reactions to our final libraries and sequenced those samples on multiple instruments. Even with this artificially high concentration of dye in the denatured library (worst case scenario), we found no effect on the sequencing run/instrument performance or the yield and quality of the final data.

Can I use other dyes besides the Green Fluorescent Dye available in the LavaLAMP™ DNA Component Kit with Dye?

PCRAMP-009, PCRAMP-010, PCRAMP-007, PCRAMP-006

Yes. Syto-13 (Molecular Probes) and EvaGreen (Biotium) have both been tested with the LavaLAMP DNA Master Mix and they perform well.

What are the storage conditions for BHQ Probe Master Mix?

BHQ-001

BHQ Probe Master Mix should be stored at -20 °C. Repeated freeze-thaws should be avoided, so if BHQ Probe Master Mix is to be aliquoted, nuclease-free, light-protected tubes/vials should be used.

Once thawed, BHQ Probe Master Mix can be stored at +4 °C for 1 week.

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