DNA and RNA Purification Kits and Reagents
Savings25
Ready-Lyse Lysozyme Solution
Product Details
ShowReady-Lyse Lysozyme Solution a ready-to-use solution supplied in quantities of 4 or 10 × 106 units.
Expert Recommendation: For difficult to lyse bacteria, Ready-Lyse Lysozyme Solution can be an additional lysis step when paired with our QuickExtract™ DNA Extraction Solution or MasterPure™ Gram Positive DNA Purification Kit, to help extract PCR-ready DNA from gram +/- bacteria or fungi. Both QuickExtract and MasterPure can be used alone or with Ready-Lyse Lysozyme Solution.
Ready-Lyse Lysozyme Solution
Ready-Lyse Lysozyme Solution is a stabilised lysozyme preparation for the lysis of Gram-negative bacteria such as E. coli, as well as Gram-positive bacteria such as Bacillus sp.
Key features
Show- Highly Active: 200X more active than egg-white lysozyme and optimally active at a lower pH of 6.5-7.5 than the standard pH 9.
- Easy: No sample agitation or heat generation necessary during lysis which helps preserve protein function
- Flexible: Usage volumes are easily adjusted based on the scale of the experiment
- Stable: stable at -20 °C, eliminating the need to prepare a fresh solution for each use
- Accurate: virtually no nonspecific binding and significantly less precipitation of DNA compared to egg-white lysozyme
Product information
Unit Definition: One unit of Ready-Lyse Lysozyme produces a decrease in A 350 of 0.001 per minute at 23 °C with a 0.5 mg/mL suspension of lyophilised E. coli K802 cells in 50 mM Tris-HCl (pH 7.5).
Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.
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