Phosphoramidite used to incorporate a 5' biotin functionality, separated from the oligonucleotide by a photo-cleavable linker.
Explore our photocleavable analogues of the Amino- and Spacer-Modifiers and the Biotin label, as well as our PC linker molecule. The general design of the PC monomers is based on an α-substituted 2-nitrobenzyl group. The photo-reactive group is derivatised as a cyanoethyl phosphoramidite that can be incorporated during automated DNA synthesis.
|PC 5'-Amino-Modifier CE-Phosphoramidite||Terminal modification that results in a 5'-monophosphate on the released oligonucleotide.||
|PC 5'-Biotin CE-Phosphoramidite||Terminal modification that results in a 5'-monophosphate on the released oligonucleotide. This phosphoramidite contains a biotinyl moiety that bears a trityl group on the N-1 nitrogen atom for N-protection.||
|PC Linker CE-Phosphoramidite||Mid-sequence modification that results in monophosphate fragments at both the 3'- and 5'-termini.||
|PC Spacer CE-Phosphoramidite||Mid-sequence modification that results in a 5'-monophosphate on the released oligonucleotide.||
Photocleavage using PC Linker Phosphoramidite
Photo-triggered DNA cleavage is an important tool used for studying conformational changes and strand breaks, as well as for studying activation of nucleic-acid-targeted drugs, such as antisense oligonucleotides.
DNA Purification using PC-5’-Biotin Phosphoramidite
This technique is commonly used to isolate DNA by first hybridisation to the biotin labelled probe, then release of the probe/DNA duplex after photolysis. After photo-cleavage the DNA is suitable for further biological manipulations like gene construction and cloning after ligation.