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NP-KLH (Keyhole Limpet Hemocyanin)

NP-KLH (Keyhole Limpet Hemocyanin)

4-Hydroxy-3-nitrophenylacetyl hapten is conjugated to KLH (Keyhole Limpet Hemocyanin) lysine through amide bonds.
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Product information

Our line of conjugates include well studied small molecule haptens eg, DNP, TNP, NP, NIP, and PC (Phosphoryl Choline). Various levels of conjugation density are available depending on the antibody response required by the researcher. Such reagents are complete antigens useful for inducing antibody response in animal models. Conjugation ratio is the molar ratio of hapten to protein. The conjugation ratio (or "loading") for any given immunochemical will vary with each production batch, and we do not offer customised ratios. Where standard ratio ranges are not offered, customer service can inform you about available ratios at the point of purchase.


  • Chemical Name: 4-Hydroxy-3-nitrophenylacetyl-Keyhole Limpet Hemocyanin
  • Appearance: yellow powder
  • Absorption Maximum (Lambda Max): 430
  • Extinction Coefficient at Lambda max: 4230

Product usage:

    Conjugation is determined at pH 8.5 by measuring NP absorbance at 430nm, using the extinction coefficient of NP= 4.23 x 10E+03. The conjugation ratio is given as a molar ratio of NP to KLH assuming the molecular weight of KLH is 100,000.Store solution in aliquots at +5°C. KLH will come out of solution if frozen. Protect from light.This product can be dissolved in PBS buffer, water or other neutral buffers at a concentration of 1mg/mL. Product may precipitate when dissolved or stored in solution, therefore it is recommended to sonicate the solution for 30 minutes before use.When using PBS please refer to the formulation below.PBS Formulation:8 g NaCl0.2 g KCl0.2 g potassium di-hydrogen phosphate (KH2PO4)1.15 g di-sodium hydrogen phosphate (Na2HPO4)Add to 1 L of water

Storage and handling:

  • Shipping conditions: Ambient
  • Storage conditions: +2 to +8 °C

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