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NP-HEL (Hen Egg Lysozyme)

NP-HEL (Hen Egg Lysozyme)

This product will be discontinued on 30 June 2024.

4-Hydroxy-3-nitrophenylacetyl hapten is conjugated to HEL (Hen Egg Lysozyme) lysine through amide bonds.

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Product information

Our line of conjugates include well studied small molecule haptens eg, DNP, TNP, NP, NIP, and PC (Phosphoryl Choline). Various levels of conjugation density are available depending on the antibody response required by the researcher. Such reagents are complete antigens useful for inducing antibody response in animal models. Conjugation ratio is the molar ratio of hapten to protein. The conjugation ratio (or "loading") for any given immunochemical will vary with each production batch, and we do not offer customised ratios. Where standard ratio ranges are not offered, customer service can inform you about available ratios at the point of purchase.

Properties:

  • Chemical Name: 4-Hydroxy-3-nitrophenylacetyl Hen Egg Lysozyme
  • Appearance: yellow powder
  • Absorption Maximum (Lambda Max): 430
  • Extinction Coefficient at Lambda max: 4230

Product usage:

    This product can be dissolved in 0.1M NaHCO3 buffer, pH 8.3-8.5 or DMSO at a concentration of 1mg/mL. Product may precipitate when stored in solution, therefore it is recommended to centrifuge the solutions briefly before use and use only the supernatant.Store solution in aliquots at -20°C. Protect from light.Conjugation is determined at pH 8.5 by measuring NP absorbance at 430 nm, using 4.23 x 10E+03 as the extinction coefficient for NP, and is given as a molar ratio of NP to HEL assuming the molecular weight of HEL is 14,300.

Storage and handling:

  • Shipping conditions: Ambient
  • Storage conditions: +2 to +8 °C

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