Fluorescein-OVAL (Ovalbumin)
Fluorescein-OVAL (Ovalbumin)
Fluorescein-OVAL (Ovalbumin), 10 mg, ABI (5 mL / 20 mm Septum)
This product will be discontinued on 30 June 2024.
5,6 Carboxyfluorescein is conjugated to Ovalbumin protein through amide bonds.
Item discontinued
Product information
Our line of conjugates include well studied small molecule haptens eg, DNP, TNP, NP, NIP, and PC (Phosphoryl Choline). Various levels of conjugation density are available depending on the antibody response required by the researcher. Such reagents are complete antigens useful for inducing antibody response in animal models. Conjugation ratio is the molar ratio of hapten to protein. The conjugation ratio (or "loading") for any given immunochemical will vary with each production batch, and we do not offer customised ratios. Where standard ratio ranges are not offered, customer service can inform you about available ratios at the point of purchase.
Properties:
- Chemical Name: Fluorescein-Ovalbumin
- Appearance: Orange powder
- Absorption Maximum (Lambda Max): 495
- Extinction Coefficient at Lambda max: 78000
Product usage:
- Conjugation is determined by measuring fluorescein absorbance at 495nm, using the extinction coefficient of fluorescein, 8.3 x 10E+04. The conjugation ratio is given as a molar ratio fluorescein to ovalbumin assuming the molecular weight of ovalbumin is 45,000.Store solution in aliquots at -20°C. Protect from light.This product can be dissolved in PBS buffer, water or other neutral buffers at a concentration of 1mg/mL. Product may precipitate when stored in solution, therefore it is recommended to centrifuge the solutions briefly before use and use only the supernatant.When using PBS please refer to the formulation below.PBS Formulation:8 g NaCl0.2 g KCl0.2 g potassium di-hydrogen phosphate (KH2PO4)1.15 g di-sodium hydrogen phosphate (Na2HPO4)Add to 1 L of water
Storage and handling:
- Shipping conditions: Ambient
- Storage conditions: +2 to +8 °C
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