5'-DMT-dU-Suc-CPG (5'-DMT-deoxyUridine CPG) Column
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5'-DMT-dU-Suc-CPG (5'-DMT-deoxyUridine CPG) Column
5'-DMT-dU-Suc-CPG (5'-DMT-deoxyUridine CPG) Column, 1000 Å, 1 µmol, Super
CPG used to incorporate a deoxyuridine base into the 3' end of an oligonucleotide.
Key features
Show- Used to induce mutagenic effects.
- The enzyme uracil-N-glycosylase (UNG) can specifically remove uracil to create abasic sites at the deoxyuridine positions. This property is used to generate site-specific strand breaks in the oligonucleotide.
- CPG has aminopropyl-succinyl linker.
- 1000 Å CPG suitable for highly modified oligonucleotides (> 20mers).
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Usually shipped in 5-10 Days, Made To Order
Product information
Incorporation of a deoxyuridine base within a DNA sequence can be used to induce mutagenic effects. The enzyme uracil-N-glycosylase (UNG) can specifically remove uracil to create abasic sites at the deoxyuridine positions. This property is used to generate site-specific strand breaks in the oligonucleotide. We provide both dU phosphoramidite and CPGs.
Synthesizer
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Column
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Type/Description
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Notes
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MerMade 6,12
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MerMade, syringe (all scales)
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Pipette type column
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A MerMade column is also known as a Supercolumn
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MerMade 4, 48X, 96E, 192E, 192X
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MerMade, Syringe (up to 1.3 mL)
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Pipette type column
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A MerMade column is also known as a Supercolumn
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ABI 384 / 394
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Luer
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Barrel column with luer fitting at either end
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Also known as ALL-FIT or Standard
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Expedite 8909
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Luer
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Barrel column with luer fitting at either end
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Also known as ALL-FIT or Standard
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ABI3900
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MerMade
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Pipette type column
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A MerMade column is also known as a Supercolumn
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K&A H4, H8, H8SE, H2, H32, H64
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Luer
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Barrel column with luer fitting at either end
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Also known as ALL-FIT or Standard
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K&A S4CL/S8CL
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Luer
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Barrel column with luer fitting at either end
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Also known as Standard. For this instrument, we recommend the Luer (Standard) column as the ALL-FIT columns have a wider barrel.
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Dr Oligo 48
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MerMade
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Pipette type column
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A MerMade column is also known as a Supercolumn
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Dr Oligo 192XLc, 768XLc just plates
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MerMade, Syringe (up to 1.3 mL)
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Pipette type column
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A MerMade column is also known as a Supercolumn
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OligoMaker X12, 48, 192, X192, X96
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MerMade, Syringe (up to 1.3 mL)
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Pipette type column
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A MerMade column is also known as a Supercolumn
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Properties:
- Appearance: white powder
Product usage:
- Cleavage conditions: Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites.
- Deprotection conditions: When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C. Image of cleaved and deprotected structure:
- The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 290.17
![](https://biosearch-cdn.azureedge.net/assetsv6/101885c.gif)
Storage and handling:
- Shipping conditions: Ambient
- Storage conditions: +2 to +8 °C in sealed container
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