Skip to content Skip to navigation menu
cancel
  1. Home
  2. PCR instruments, reagents and consumables
  3. PCR and qPCR Reagents
  4. PCR and qPCR Enzymes and Reagents
  5. FailSafe Enzyme Mix

Savings25

FailSafe Enzyme Mix

FailSafe Enzyme Mix, 2.5 U/µL, 100 U

Product Details

Show Hide

The PCR Enzyme Mix is a blend of thermostable DNA polymerases containing a 3′→5′ proofreading enzyme for high fidelity. Supplied in a 50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 0.5% Tween®20, 0.5% NP40, and 1 mM dithiothreitol.

Choose from 2 different sizes of FailSafe PCR Enzyme Mix, 100 Units (40 uL) and 1000 Units (400 uL).

Before purchasing we strongly recommend first optimising your PCR conditions using the FailSafe PCR PreMix Selection Kit. If optimization is complete, you can proceed with the bundled and affordable post optimisation FailSafe PCR System.

The “failsafe” nature is guaranteed when using all of the FailSafe components.

FailSafe Enzyme Mix

FailSafe Enzyme Mix, 2.5 U/µL, 100 U

PCR Enzyme Mix, individual component of the FailSafe PreMix Selection Kit for PCR optimization, and the bundled post - optimization FailSafe PCR System.

Key features

Show Hide
  • Unique blend of thermostable enzymes
  • 3′→5′ proofreading enzyme
  • High fidelity at least 3 times higher than Taq DNA polymerase
Select a Size
Item ID FSE51100
TBD
Add to basket to request a quote

Similar Products

FailSafe PCR PreMix Selection Kit

PCR and qPCR Enzymes and Reagents

FailSafe PCR PreMix Selection Kit

PCR optimisation kit for end-point applications. Includes everything needed to optimise PCR performance and achieve consistent, high-fidelity results for every DNA template…

ITEM ID
FS99060
View
FailSafe PCR 2X PreMixes

PCR and qPCR Enzymes and Reagents

FailSafe PCR 2X PreMixes

Twelve 2X PreMixes, individual components of the FailSafe PreMix Selection Kit for PCR optimization, and the bundled post - optimization FailSafe PCR System.

View
Added to basket

Product information

This is the standalone Enzyme Mix component of the FailSafe PCR PreMix Selection Kit for optimization, and the post optimisation FailSafe PCR System.

The Enzyme Mix is a unique blend of thermostable DNA polymerases, containing a 3′→5′ proofreading enzyme which helps achieve fidelity at least 3 times higher than Taq DNA polymerase. The thermostable enzymes may help against DNA denaturation when exposed to high temperatures, and may have a tolerance against certain inhibitors, making them ideal enzymes for every PCR need.1

Simply add your template, primers, and the FailSafe PCR Enzyme Mix to each of the FailSafe 2X PreMixes and amplify.

For a complete explanation of how FailSafe technology works, please read the manual.

Reference

           1. Killelea T, Ralec C, Bossé A, Henneke G. PCR performance of a thermostable heterodimeric archaeal DNA polymerase. Front Microbiol. 2014 May 7;5:195. doi: 10.3389/fmicb.2014.00195. PMID: 24847315; PMCID: PMC4019886.

SDS

Manuals and user guides

Product information sheets

You may also be interested in...

RapiDxFire Hot Start Taq DNA Polymerase

Concentration

RapiDxFire Hot Start Taq DNA Polymerase

Reliable hot start Taq DNA Polymerase for sensitive detection of low abundance target DNA. Ideal for standard end point PCR and qPCR applications. Available in Glycerol-free, …
View
Added to basket

Access support

Need some support with placing an order, setting up an account, or finding the right protocol?

Contact us