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NxGen M-MuLV Reverse Transcriptase

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Supplied at 200,000 U/mL in 50 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.1% NP-40 Alternative, 50% glycerol, pH 7.6 @ 25 °C. Also provided is 10X M-MuLV RT Buffer which in 1X form is composed of 50 mM Tris-HCl, 75 mM KCl, 3 mM MgCl2, 10 mM dithiothreitol, pH 8.3 @ 25 °C. 250,000 U is supplied as 5 × 50,000 U.

NxGen M-MuLV Reverse Transcriptase

Outstanding cDNA synthesis.

Size: 50,000 U

Item ID 30222-1
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Product information

M-MuLV Reverse Transcriptase is an RNA-dependent DNA polymerase which shows no measurable 3′→5′ proofreading function. This enzyme can copy a single-stranded DNA template or perform cDNA synthesis by extending a DNA primer annealed to an RNA template.

Unit Definition: 1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37 °C using poly r(A)/oligo (dT) as a substrate.

Source: A recombinant E. coli strain carrying the Moloney-Murine Leukemia Virus Reverse Transcriptase gene.

Unit Concentration 200,000 U/mL
Purity (SDS-PAGE) >99%
SS Exonuclease 2,000 U <5.0% released
DS Exonuclease 2,000 U <0.5% released
Endonuclease 2,000 U <10% converted
E. coli 16S rDNA Contamination 2,000 U <10 copies
Storage -20 °C

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