Reverse Transcriptases
EpiScript RNase H- Reverse Transcriptase Kit
Product Details
ShowKit Contents: EpiScript RNase H- Reverse Transcriptase (200 U/µL), 10X Reaction Buffer, 100 mM DTT.
EpiScript RNase H- Reverse Transcriptase Kit
EpiScript RNase H- Reverse Transcriptase Kit, 200 U/µL, 10,000 U
Key features
Show- Produce full-length cDNA with RNAse H-mutant
- Active at elevated temperatures up to 55 °C
Product information
EpiScript RNase H-- Reverse Transcriptase (EpiScript RT), is a recombinant MMLV reverse transcriptase with greatly reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. EpiScript RT is capable of producing cDNA from as little as 50 pg of total RNA for real-time RT-PCR (qRT-PCR) analysis and other applications.
Applications
- First-strand cDNA synthesis for subsequent PCR or real-time PCR.
Benefits
- Recombinant MMLV reverse transcriptase with greatly reduced RNase H activity
- Active at temperatures up to 55 °C
- Highly efficient at producing full-length cDNAs
Storage: Store only at -20 °C in a freezer without a defrost cycle.
Storage Buffer: EpiScript RT is supplied in a 50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 100 mM sodium chloride, 1 mM DTT, 0.1 mM EDTA, and 0.1% Triton® X-100.
Unit Definition: One unit of EpiScript RT catalyses the incorporation of 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37 °C using saturating amounts of oligo(dT)-primed poly(rA) as template.
Contaminating Activity Assays: EpiScript RT is free of detectable exonuclease, endonuclease, and RNase activities.
SDS
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ERT12925K EpiScript RNase H Reverse Transcriptase
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ERT12925K 1 EpiScript RNase H Reverse Transcriptase English US
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