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EpiScript RNase H- Reverse Transcriptase Kit

Kit Contents: EpiScript RNase H- Reverse Transcriptase, 10X Reaction Buffer, 100 mM DTT.

EpiScript RNase H- Reverse Transcriptase Kit

An optimised reverse transcriptase system for the production of full-length cDNA.
  • Produce full-length cDNA with RNAse H-mutant
  • Achieve higher specificity at elevated temperatures, up to 55 °C
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Product information

EpiScript RNase H-- Reverse Transcriptase (EpiScript RT), is a recombinant MMLV reverse transcriptase with greatly reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. EpiScript RT is capable of producing cDNA from as little as 50 pg of total RNA for real-time RT-PCR (qRT-PCR) analysis and other applications.

Applications

  • First-strand cDNA synthesis for subsequent PCR or real-time PCR.

Benefits

  • Recombinant MMLV reverse transcriptase with greatly reduced RNase H activity
  • Active at temperatures up to 55 °C
  • Highly efficient at producing full-length cDNAs

Storage: Store only at -20 °C in a freezer without a defrost cycle.

Storage Buffer: EpiScript RT is supplied in a 50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 100 mM sodium chloride, 1 mM DTT, 0.1 mM EDTA, and 0.1% Triton® X-100.

Unit Definition: One unit of EpiScript RT catalyses the incorporation of 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37 °C using saturating amounts of oligo(dT)-primed poly(rA) as template.

Contaminating Activity Assays: EpiScript RT is free of detectable exonuclease, endonuclease, and RNase activities.

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