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FailSafe PCR PreMix Selection Kit

Includes FailSafe PCR Enzyme Mix (60 U) and all 12 FailSafe PCR 2X PreMixes (100 µL each).

FailSafe PCR PreMix Selection Kit

Dependable, consistent high-fidelity PCR results with every target DNA template.
  • PCR amplification of difficult or high-GC templates
  • Multiplex PCR compatible
  • Robust amplification of targets up to 20 kb long
  • High accuracy with 3-fold lower error rate than Taq DNA polymerase (error rate = 1 in 30,000)
  • Extremely high sensitivity and specificity using the PCR Enhancer Technology (with Betaine)

Size: 60 U

Item ID FS99060
TBD
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Product information

The FailSafe™ PCR System provides dependable, consistent high-fidelity PCR results for every DNA template, regardless of its source or sequence. The FailSafe™ PCR System will faithfully amplify your template every time.

Applications

  • End-point PCR optimisation
  • Consistent and reproducible PCR

How the FailSafe System Works

  1. First, purchase the FailSafe PCR PreMix Selection Kit (catalogue number FS99060). The FailSafe PCR PreMix Selection Kit is composed of:
    • FailSafe PCR Enzyme Blend (60 Units)
    • Twelve (12) different FailSafe™ 2X PCR PreMixes. Each 2X PreMix contains dNTPs, Buffer and different concentrations of Mg+2 and Epicentre's proprietary PCR Enhancer (with Betaine).
  2. Prepare and perform PCR reactions using your template and PCR primers with each of the 12 FailSafe™ 2X PCR PreMixes and the FailSafe PCR Enzyme.
  3. Analyse, by agarose gel electrophoresis, the PCR product produced from each of the 12 reactions and visually determine which PreMix gives the best amplification (e.g., a single, strong PCR product of the desired length or "as expected" multiplex PCR results). We guarantee that one of the FailSafe PCR 2X PreMixes will give the desired amplification product.
  4. For reliable and consistent PCR results, use the FailSafe PCR Enzyme and the best 2X PreMix identified in the optimisation experiments for all subsequent amplifications with that template/primer combination.
  5. To reduce costs, purchase a FailSafe PCR System (Item No. FS99100, FS99250,or FS9901K) which provides sufficient enzyme and the appropriate amount of your favourite 2X PCR Premix(es) instead of buying the enzyme and 2X PCR Premixes separately.
Figure 1. Amplification of an 80%-85% GC-rich region of the human fragile X gene. PCR was performed using the FailSafe™ PCR System, schematically depicted above. Lanes A-L show the amplification products resulting from PCR using the 12 FailSafe PCR PreMixes. Lane M, molecular weight marker. In this experiment, optimal amplification was obtained with FailSafe PCR PreMix J. The size of the expected amplicon is indicated by an arrow. Figure 2. Multiplex PCR of the human CFTR gene. The FailSafe™ PCR System amplified all five exons of the CFTR gene from as little as 1 ng of human genomic DNA. L

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