DNA and RNA Purification Kits and Reagents
Proteinase K
Digest proteins during DNA and RNA purification or inactivate enzymes. To be used with the MasterPure Kits.
MasterPure Yeast RNA Purification Kit
Product Details
ShowContents: Extraction Reagent for RNA, MPC Protein Precipitation Reagent, TE Buffer (in 100-reaction kit only), Proteinase K, RNase-Free DNase I, RiboGuard RNase Inhibitor, 10X DNase Buffer, 2X T & C Lysis Solution.
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MasterPure Yeast RNA Purification Kit
Safe, fast purification of high quality RNA from multiple species of yeast.
Key features
Show- Safe, fast purification of high quality RNA from multiple species of yeast
- Safe: Avoids the use of dangerous hot acid phenol
- Fast: Quicker protocol that methods using spheroplasting
- High Quality RNA: RNA quality exceeds that obtained by bead beating protocols and is ready for use in downstream applications such and RT-PCR and microarray analysis
- Excellent Yields: Yields 25-50 µg RNA from 1 ml of mid-log S.cerevisiae
- Complete: All the necessary reagents are supplied with no need to purchase extra equipment
Number of purifications: 100
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Tissue & Cell Lysis Solution
Use in combination with Proteinase K to lyse cells and tissues in various samples such as saliva, semen, mammalian cells, and Gram-negative bacteria.
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Product information
The MasterPure™ Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from yeast cell types (including Candida, Saccharomyces, Schizosaccharomyces) and filamentous fungi. The kit uses a rapid salting-out process1 to remove contaminating macromolecules, avoiding toxic organic solvents, bead-beating, and spheroplasting.2
References
- Miller, S.A. et al., (1988) Nucleic Acids Res. 16, 1215.
- Hoffman, L.M. and Jarvis, B.W. (2004) Epicentre Forum 11(2), 4.
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| Figure 1. Microarray analysis of yeast RNA purified with the MasterPure™ Yeast RNA Purification Kit. The RNA was reverse transcribed, labelled, and hybridised to a S. cerevisiae DNA microarray. The cDNA from cells under oxidative stress was labelled with Cy5, and cDNA from control cells was labelled with Cy3. | Figure 2. Purity of RNA obtained using the MasterPure™ Yeast RNA Purification Kit. The RNA used in Figure. 1. was stored at -20 °C for two months before analysis on an Agilent Technologies 2100 Bioanalyzer®. The electrophoretogram demonstrates the high-purity, intact RNA. |
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