5'-DMT-rG (Pac)-Suc CPG Column
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5'-DMT-rG (Pac)-Suc CPG Column
CPG column for incorporation of unmodified ribo-G at 3' end of an oligonucleotide.
Key features
Show- CPG has aminopropyl succinyl linker
- Phenoxyacetyl (Pac) protection for use in UltraMILD conditions
- Standard luer or Supercolumn pipette column types available
- Available in different synthesis scales and pore sizes
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Product information
Various RNA CPG column formats are available, and compatible with a range of instruments.
Column Type | Description | Compatible synthesizers |
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Hybrid columns |
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Supercolumns or MerMade columns |
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Standard or ALL-FIT columns |
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TWIST™ columns |
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Properties:
- Appearance: White Powder
Product usage:
- Synthesis conditions: Follow the instrument manufacturer's protocol when using this product. If using PAC protected supports or reagents we recommend using phenoxyacetic anhydride or t.butyl phenoacetal acid anhydride(FAST deprotecting capping) as the CAP A reagent. Standard acetic anhydride (CAP A) can cause acetylation of the PAC protected primary amines during synthesis.
- Cleavage conditions: Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites.We do not recommend using AMA to cleave or deprotect sequences that contain uridine. The AMA may degrade the uridine nucleobase.
- Deprotection conditions: When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.
- The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 345.2
Storage and handling:
- Shipping conditions: Ambient
- Storage conditions: -15 to -30 °C in sealed container
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