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  6. 5'-DMT-rG (Pac)-Suc CPG Column

5'-DMT-rG (Pac)-Suc CPG Column

5'-DMT-rG (Pac)-Suc CPG Column
5'-DMT-rG (Pac)-Suc CPG Column
5'-DMT-rG (Pac)-Suc CPG Column
5'-DMT-rG (Pac)-Suc CPG Column
5'-DMT-rG (Pac)-Suc CPG Column

5'-DMT-rG (Pac)-Suc CPG Column

CPG column for incorporation of unmodified ribo-G at 3' end of an oligonucleotide.

Key features

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  • CPG has aminopropyl succinyl linker
  • Phenoxyacetyl (Pac) protection for use in UltraMILD conditions
  • Standard luer or Supercolumn pipette column types available
  • Available in different synthesis scales and pore sizes
Option 1: Select a Pore Size
Option 2: Select a Column Type
Option 3: Select a Scale
TBD
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Product information

Various RNA CPG column formats are available, and compatible with a range of instruments. 

Synthesizer
Column
Type/Description
Notes
MerMade 6,12
MerMade, syringe (all scales)
Pipette type column
A MerMade column is also known as a Supercolumn
MerMade 4, 48X, 96E, 192E, 192X
MerMade, Syringe (up to 1.3 mL)
Pipette type column
A MerMade column is also known as a Supercolumn
ABI 384 / 394
Luer
Barrel column with luer fitting at either end
Also known as ALL-FIT or Standard
Expedite 8909
Luer
Barrel column with luer fitting at either end
Also known as ALL-FIT or Standard
ABI3900
MerMade
Pipette type column
A MerMade column is also known as a Supercolumn
K&A H4, H8, H8SE, H2, H32, H64
Luer
Barrel column with luer fitting at either end
Also known as ALL-FIT or Standard
K&A S4CL/S8CL
Luer
  Barrel column with luer fitting at either end 
 Also known as Standard. For this instrument, we recommend the Luer (Standard) column as the ALL-FIT columns have a wider barrel.
Dr Oligo 48
MerMade
Pipette type column
A MerMade column is also known as a Supercolumn
 Dr Oligo 192XLc, 768XLc just plates 
 MerMade, Syringe (up to 1.3 mL) 
Pipette type column
A MerMade column is also known as a Supercolumn
 OligoMaker X12, 48, 192, X192, X96 
MerMade, Syringe (up to 1.3 mL)
Pipette type column
A MerMade column is also known as a Supercolumn

Properties:

  • Appearance: White Powder

Product usage:

  • Synthesis conditions: Follow the instrument manufacturer's protocol when using this product. If using PAC protected supports or reagents we recommend using phenoxyacetic anhydride or t.butyl phenoacetal acid anhydride(FAST deprotecting capping) as the CAP A reagent. Standard acetic anhydride (CAP A) can cause acetylation of the PAC protected primary amines during synthesis.
  • Cleavage conditions: Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites.We do not recommend using AMA to cleave or deprotect sequences that contain uridine. The AMA may degrade the uridine nucleobase.
  • Deprotection conditions: When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.
  • The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 345.2

Storage and handling:

  • Shipping conditions: Ambient
  • Storage conditions: -15 to -30 °C in sealed container

SDS

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