N Mix Suc-CPG Column (5'-DMT-[dA(Bz), dC(Ac), dG(iBu), T]-Suc-CPG)
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N Mix Suc-CPG Column (5'-DMT-[dA(Bz), dC(Ac), dG(iBu), T]-Suc-CPG)
CPG synthesis column for incorporation of mixed unmodified dN at 3' end of an oligonucleotide.
Key features
Show- CPG mix has glycolate linkers for use with mild deprotection conditions
- Standard luer column type available in different synthesis scales
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Product information
Various DNA CPG column formats are available, and compatible with a range of instruments.
Synthesizer
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Column
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Type/Description
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Notes
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---|---|---|---|
MerMade 6,12
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MerMade, syringe (all scales)
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Pipette type column
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A MerMade column is also known as a Supercolumn
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MerMade 4, 48X, 96E, 192E, 192X
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MerMade, Syringe (up to 1.3 mL)
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Pipette type column
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A MerMade column is also known as a Supercolumn
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ABI 384 / 394
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Luer
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Barrel column with luer fitting at either end
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Also known as ALL-FIT or Standard
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Expedite 8909
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Luer
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Barrel column with luer fitting at either end
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Also known as ALL-FIT or Standard
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ABI3900
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MerMade
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Pipette type column
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A MerMade column is also known as a Supercolumn
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K&A H4, H8, H8SE, H2, H32, H64
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Luer
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Barrel column with luer fitting at either end
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Also known as ALL-FIT or Standard
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K&A S4CL/S8CL
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Luer
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Barrel column with luer fitting at either end
|
Also known as Standard. For this instrument, we recommend the Luer (Standard) column as the ALL-FIT columns have a wider barrel.
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Dr Oligo 48
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MerMade
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Pipette type column
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A MerMade column is also known as a Supercolumn
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Dr Oligo 192XLc, 768XLc just plates
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MerMade, Syringe (up to 1.3 mL)
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Pipette type column
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A MerMade column is also known as a Supercolumn
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OligoMaker X12, 48, 192, X192, X96
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MerMade, Syringe (up to 1.3 mL)
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Pipette type column
|
A MerMade column is also known as a Supercolumn
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Product usage:
- Cleavage conditions: Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites.
- Deprotection conditions: When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.
Storage and handling:
- Shipping conditions: Ambient
- Storage conditions: +2 to +8 °C in sealed container
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