Controlled Pore Glass (CPG) has been widely used as a solid support for oligo synthesis for several decades. LGC, Biosearch Technologies’ has perfected CPG manufacture for maximum oligo purity and yield. Our advanced CPG production techniques were developed originally in our Prime Synthesis business to improve control of particle size and shape, pore size, pore volume, and specific surface area. These physical parameters influence solution exchange behaviour, ligand loading and distribution, and reaction kinetics to increase the efficiency, purity, and reproducibility of syntheses. Proprietary chemical attachment procedures were developed to further optimise ligand distributions, providing increased accessibility to synthesis reagents and washing solutions and facilitating even better oligo yields and purities. Furthermore, process refinements and proprietary assays were developed to minimise the troublesome “N-1” impurity levels in an oligo synthesis. LGC, Biosearch Technologies’ CPG is considered to be the gold-standard solid support used in all sectors of the market.

Our collaborative process has resulted in solid supports that are optimised for the synthesis of the latest therapeutic oligo classes including LNA, delivery enhancing lipid ligands, siRNA and Spiegelmers. Our CPG solid supports are available in a variety of pore sizes and functionalised nucleoside loadings. Which pore size and loading required is dependent on the length, complexity and application of the oligo. 500 Å and 600 Å CPG is suited to ≤30mers in medium to large scale oligo synthesis, where high yields of product are required such as for therapeutic oligos. 500 Å CPG can load up to ~100 μmol/g. 1000 Å CPG is suited to >20mers synthesis or for highly modified oligonucleotides. Most of our modifier CPGs are functionalised onto this pore size as standard. 2000 Å CPG offers the ability to synthesise longers oligos, whilst retaining higher loading (yield) possibilites of lower pore sizes. 2000 Å CPG is typically used in CRISPR applications. 3000 Å CPG is suited to longer oligos (>80mers). With a few exceptions, it is possible for us to manufacture any of our 1000 Å products with a 3000 Å pore size on a custom basis. Our Prime Synthesis bulk CPGs are manufactured with either an aminopropyl (AMP) or long-chain alkyl amino (CNA) linker, denoted in the product name.