5'-DMT-T-Suc CPG Column
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5'-DMT-T-Suc CPG Column
CPG synthesis column for incorporation of unmodified dT at 3' end of an oligonucleotide.
Key features
Show- CPG has aminopropyl-succinyl linker
- Standard luer or Supercolumn pipette column types available in different synthesis scales
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Product information
Various DNA CPG column formats are available, and compatible with a range of instruments.
Column Type | Description | Compatible synthesizers |
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Hybrid columns |
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Supercolumns or MerMade columns |
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Standard or ALL-FIT columns |
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TWIST™ columns |
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Properties:
- Appearance: White Powder
- Extinction Coefficient at 260 nm: 8400
Spectral properties measured in water, for the cleaved and deprotected nucleoside.
Product usage:
- Cleavage conditions: Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites.
- Deprotection conditions: When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C. ABI 3900 users: Columns should be compatible with standard ABI protocols. However, when using 50 nmol Super Columns, the following changes are recommended: 1) Change the head pressure/purge pressure/chamber pressure to 3.5 psi.2) An additional oxidation step3) OPTIONAL (but may help): extension of the coupling time to 20 secImage of cleaved and deprotected structure:
- The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 304.19

Storage and handling:
- Shipping conditions: Ambient
- Storage conditions: +2 to +8 °C in sealed container
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