5'-DMT-dG (iBu)-Suc-CPG Column
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5'-DMT-dG (iBu)-Suc-CPG Column
CPG synthesis column for incorporation of unmodified dG at 3' end of an oligonucleotide.
Key features
Show- CPG has aminopropyl-succinyl linker
- Standard luer or Supercolumn pipette column types available in different synthesis scales
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Product information
Various DNA CPG column formats are available, and compatible with a range of instruments.
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Synthesizer
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Column
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Type/Description
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Notes
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MerMade 6,12
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MerMade, syringe (all scales)
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Pipette type column
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A MerMade column is also known as a Supercolumn
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MerMade 4, 48X, 96E, 192E, 192X
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MerMade, Syringe (up to 1.3 mL)
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Pipette type column
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A MerMade column is also known as a Supercolumn
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ABI 384 / 394
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Luer
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Barrel column with luer fitting at either end
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Also known as ALL-FIT or Standard
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Expedite 8909
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Luer
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Barrel column with luer fitting at either end
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Also known as ALL-FIT or Standard
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ABI3900
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MerMade
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Pipette type column
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A MerMade column is also known as a Supercolumn
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K&A H4, H8, H8SE, H2, H32, H64
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Luer
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Barrel column with luer fitting at either end
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Also known as ALL-FIT or Standard
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K&A S4CL/S8CL
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Luer
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Barrel column with luer fitting at either end
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Also known as Standard. For this instrument, we recommend the Luer (Standard) column as the ALL-FIT columns have a wider barrel.
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Dr Oligo 48
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MerMade
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Pipette type column
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A MerMade column is also known as a Supercolumn
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Dr Oligo 192XLc, 768XLc just plates
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MerMade, Syringe (up to 1.3 mL)
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Pipette type column
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A MerMade column is also known as a Supercolumn
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OligoMaker X12, 48, 192, X192, X96
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MerMade, Syringe (up to 1.3 mL)
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Pipette type column
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A MerMade column is also known as a Supercolumn
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Properties:
- Appearance: White Powder
- Extinction Coefficient at 260 nm: 12010
Spectral properties measured in water, for the cleaved and deprotected nucleoside.
Product usage:
- Cleavage conditions: Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites.
- Deprotection conditions: When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C. ABI 3900 users: Columns should be compatible with standard ABI protocols. However, when using 50 nmol Super Columns, the following changes are recommended: 1) Change the head pressure/purge pressure/chamber pressure to 3.5 psi.2) An additional oxidation step3) OPTIONAL (but may help): extension of the coupling time to 20 secImage of cleaved and deprotected structure:
- The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 329.2
Storage and handling:
- Shipping conditions: Ambient
- Storage conditions: +2 to +8 °C in sealed container
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