5'-DMT-dC (Bz)-Suc-CPG Column
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5'-DMT-dC (Bz)-Suc-CPG Column
CPG synthesis column for incorporation of unmodified dC at 3' end of an oligonucleotide.
Key features
Show- CPG has aminopropyl-succinyl linker
- Standard luer or Supercolumn pipette column types available in different synthesis scales
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Product information
Various DNA CPG column formats are available, and compatible with a range of instruments.
Column Type | Description | Compatible synthesizers |
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Hybrid columns |
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Supercolumns or MerMade columns |
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Standard or ALL-FIT columns |
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TWIST™ columns |
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Properties:
- Appearance: white powder
- Extinction Coefficient at 260 nm: 7050
Spectral properties measured in water, for the cleaved and deprotected nucleoside.
Product usage:
- Cleavage conditions: Cleave in concentrated ammonia for 90 minutes at 25°C.
- Deprotection conditions: Deprotect in concentrated ammonia for 5 hours at 60 °C.Use only concentrated NH4OH only with this product. ABI 3900 users: Columns should be compatible with standard ABI protocols. However, when using 50 nmol Super Columns, the following changes are recommended: 1) Change the head pressure/purge pressure/chamber pressure to 3.5 psi.2) An additional oxidation step3) OPTIONAL (but may help): extension of the coupling time to 20 secImage of cleaved and deprotected structure:
- The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 289.18

Storage and handling:
- Shipping conditions: Ambient
- Storage conditions: +2 to +8 °C in sealed container
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