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7-Methyl-6-thioguanosine (MESG)

7-Methyl-6-thioguanosine (MESG)

CAS No.: 55727-10-1

Useful phophate assay.
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7-Methyl-6-thioguanosine (MESG, Abs = 330 nm) forms 7-methyl-6-thioguanine (Abs = 355 nm) in the presense of inorganic phosphate and purine nucleoside phosphorylase (PNP) and thus constitutes a rapid and sensitive assay for phosphate. If inorganic pyrophosphatase is present, MESG may also be used to detect inorganic pyrophosphate.(1) MESG should be used as soon as possible after dissolution.

Ref:

  1. (a) Webb, M. R. PNAS 1992, 89, 4884-4887. (b) Wedler, F. C.; Ley, B. W.; Moyer, M. L. Anal. Biochem. 1994, 218, 449-453. (c) Cheng, J.; Farutin, V.; Wu, Z.; Jacob-Mosier, G.; Riley, B.; Hakimi, B.; Cordes, E. H. Bioorg. Chem. 1999, 27, 307-325.
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