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4-Thio-dT CE-Phosphoramidite

4-Thio-dT CE-Phosphoramidite

CAS No.:1290537-73-3

Phosphoramidite for incorporation of a 4-thio-modified deoxythymidine in a synthetic oligonucleotide.
  • Useful for photo cross-linking and photo-affinity labelling applications.
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Product information

Sulphur modified bases are of particular use for crosslinking. 4-Thio-dT-CE Phosphoramidite provides a convenient modification for photo cross-linking and photo-affinity labelling applications.

Applicable Products

LK2069 2-Aminopurine-CE Phosphoramidite
LK2070 4-Thio-dT-CE Phosphoramidite

Physical & Dilution Data

Dilution volumes (in ml) are for 0.1M solutions in dry acetonitrile (LK4050). Adjust accordingly for other concentrations. For µmol pack sizes, products should be diluted as 100µmol/ml to achieve 0.1M, regardless of molecular weight.

Item

Mol. Formula

Mol. Wt.

Unit Wt.

250mg

500mg

1g

LK2069 C43H53N8O6P 808.93 313.21 3.09 6.18 12.36
LK2070 C43H52N5O7PS 813.95 320.26 3.07 6.14 12.29

Coupling

No changes are required from the standard method recommended by the synthesiser manufacturer. Coupling is as per standard nucleoside amidites.

Oxidation

0.02M Oxidiser is recommended for LK2070.

Deprotection

Use standard deprotection conditions with LK2069. For LK2070 it is desirable to remove the cyanoethyl protecting group using 1M DBU in acetonitrile for 3h at room temperature, prior to cleavage and deprotection. The latter is achieved with 50mM NaSH in ammonium hydroxide solution for 24h at room temperature.

Storage & Stability

Refrigerate dry compounds. Stability in solution is 2-3 days.

 

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