4-Thio-dT CE-Phosphoramidite
4-Thio-dT CE-Phosphoramidite
CAS No.:1290537-73-3
Key features
Show- Useful for photo cross-linking and photo-affinity labelling applications.
Product information
Sulphur modified bases are of particular use for crosslinking. 4-Thio-dT-CE Phosphoramidite provides a convenient modification for photo cross-linking and photo-affinity labelling applications.
Applicable Products
| LK2069 | 2-Aminopurine-CE Phosphoramidite |
| LK2070 | 4-Thio-dT-CE Phosphoramidite |
Physical & Dilution Data
Dilution volumes (in ml) are for 0.1M solutions in dry acetonitrile (LK4050). Adjust accordingly for other concentrations. For µmol pack sizes, products should be diluted as 100µmol/ml to achieve 0.1M, regardless of molecular weight.
Item |
Mol. Formula |
Mol. Wt. |
Unit Wt. |
250mg |
500mg |
1g |
| LK2069 | C43H53N8O6P | 808.93 | 313.21 | 3.09 | 6.18 | 12.36 |
| LK2070 | C43H52N5O7PS | 813.95 | 320.26 | 3.07 | 6.14 | 12.29 |
Coupling
No changes are required from the standard method recommended by the synthesiser manufacturer. Coupling is as per standard nucleoside amidites.
Oxidation
0.02M Oxidiser is recommended for LK2070.
Deprotection
Use standard deprotection conditions with LK2069. For LK2070 it is desirable to remove the cyanoethyl protecting group using 1M DBU in acetonitrile for 3h at room temperature, prior to cleavage and deprotection. The latter is achieved with 50mM NaSH in ammonium hydroxide solution for 24h at room temperature.
Storage & Stability
Refrigerate dry compounds. Stability in solution is 2-3 days.
Access support
Need some support with placing an order, setting up an account, or finding the right protocol?
Contact us