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  2. Nucleic Acid Chemistry Reagents and Instruments
  3. Modified Oligo Synthesis Reagents
  4. Structural Studies
  5. Duplex Modifiers
  6. Propyne dU Amidite

Propyne dU Amidite

Propyne dU Amidite

Propyne dU Amidite

Phosphoramidite used to incorporate a C-5 propyne modified dU either internally or at the 5' end of an oligonucleotide.
  • Enhances base pairing.
  • Confers duplex stability and melting temperatures are raised by 1.7° per substitution.
  • Confers nuclease resistance and binding affinity of antisense oligos.
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Product information

Substitution of C-5 propynyl-dC (pdC) for dC and C-5 propynyl-dU (pdU) for dT are effective strategies to enhance base pairing. Duplex stability and melting temperatures are raised by 2.8° or 1.7° per substitution of pdC or pdU respectively. Such Tm modification is used in Turbo Taqman probes for example. C5-propyne pyrimidine modifications are sometimes added to increase nuclease resistance and binding affinity of antisense oligos.

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