Skip to content Skip to navigation menu
cancel
  1. Home
  2. Nucleic Acid Chemistry Reagents and Instruments
  3. Modified Oligo Synthesis Reagents
  4. Structural Studies
  5. Duplex Modifiers
  6. Propyne dU Amidite

Propyne dU Amidite

Propyne dU Amidite

Propyne dU Amidite

Phosphoramidite used to incorporate a C-5 propyne modified dU either internally or at the 5' end of an oligonucleotide.

Key features

Show Hide
  • Enhances base pairing.
  • Confers duplex stability and melting temperatures are raised by 1.7° per substitution.
  • Confers nuclease resistance and binding affinity of antisense oligos.
Option 1: Select a Size
Option 2: Select a Bottle Type
TBD
Add to basket to request a quote

Product information

Substitution of C-5 propynyl-dC (pdC) for dC and C-5 propynyl-dU (pdU) for dT are effective strategies to enhance base pairing. Duplex stability and melting temperatures are raised by 2.8° or 1.7° per substitution of pdC or pdU respectively. Such Tm modification is used in Turbo Taqman probes for example. C5-propyne pyrimidine modifications are sometimes added to increase nuclease resistance and binding affinity of antisense oligos.

Product usage:

    Image of cleaved and deprotected structure:

Access support

Need some support with placing an order, setting up an account, or finding the right protocol?

Contact us