dC (Ac) (5-Me) CE-Phosphoramidite
dC (Ac) (5-Me) CE-Phosphoramidite
Key features
Show- C-5 Methyl pyrimidine nucleosides are known to stabilise duplexes relative to the non-methylated bases.
- The stabilisation properties of Me-dC make this a suitable modification for stabilisation of triplex strands, where its presence raises the melting temperature of the third strand.
- Acetyl nucleobase protection allows compability with milder deprotection conditions.
Product information
The hybridisation properties of synthetic oligonucleotides are crucial for almost all applications. Optimisation of base pairing, and subsequent duplex stabilisation, is therefore desirable. C-5 Methyl pyrimidine nucleosides are known to stabilise duplexes relative to the non- methylated bases. Therefore the use of 5-Me-dC-CE Phosphoramidite (available either as N-Bz or N-Ac )(1) rather than dC results in enhanced binding (a similar comparison can be made between thymidine and 2'-deoxyuridine). This increase in duplex stabilisation is attributed to the hydrophobic nature of the methyl groups that helps eliminate water molecules from the duplex. We also offer a 5-Me-dC CPG for modification at the 3'-end. The stabilisation properties of Me-dC make this a suitable modification for stabilisation of triplex strands, where its presence raises the melting temperature of the third strand.
Ref:
- Effect of 5-methylcytosine on the stability of triple-stranded DNA-a thermodynamic study, L.E. Xodo, G. Manzini, F. Quadrifoglio, G.A. van der Marel and J.H. van Boom, Nucleic Acids Research, 19, 5625-5631, 1991.
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