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5'-DMT-dU-Suc-CPG (5'-DMT-deoxyUridine CPG)

5'-DMT-dU-Suc-CPG (5'-DMT-deoxyUridine CPG)

CPG used to incorporate a deoxyuridine base into the 3' end of an oligonucleotide.
  • Used to induce mutagenic effects.
  • The enzyme uracil-N-glycosylase (UNG) can specifically remove uracil to create abasic sites at the deoxyuridine positions. This property is used to generate site-specific strand breaks in the oligonucleotide.
  • CPG has aminopropyl-succinyl linker.
  • 1000 Å CPG suitable for highly modified oligonucleotides (> 20mers).
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Product information

Incorporation of a deoxyuridine base within a DNA sequence can be used to induce mutagenic effects. The enzyme uracil-N-glycosylase (UNG) can specifically remove uracil to create abasic sites at the deoxyuridine positions. This property is used to generate site-specific strand breaks in the oligonucleotide. We provide both dU phosphoramidite and CPGs.

Properties:

  • Appearance: white powder

Product usage:

  • Cleavage conditions: Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites.
  • Deprotection conditions: When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.
  • Image of cleaved and deprotected structure:
  • The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 290.17

Storage and handling:

  • Shipping conditions: Ambient
  • Storage conditions: +2 to +8 °C

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