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8-oxo-dG(iBu) CE-Phosphoramidite

8-oxo-dG(iBu) CE-Phosphoramidite

CAS No.:143060-53-1

Phosphoramidite used to incorporate a 8-oxo-modified deoxyguanosine into an oligonucleotide.

Key features

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  • Useful for mutagenic studies involving oxidative processes or ionisation radiation.
  • Classical isobutyryl protection.
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Product information

8-oxo-dG-CE Phosphoramidite allows investigation of the structure and activity of oligonucleotides containing an 8-oxo mutation. This is formed naturally when DNA is subjected to oxidative conditions or ionising radiation. The resulting 8-oxo modification is significant in mutagenesis and ultimately carcinogenesis.

Applicable Products

LK2072 8-oxo-dG-CE Phosphoramidite

Physical & Dilution Data

Dilution volumes (in ml) are for 0.1M solutions in dry acetonitrile (LK4050). Adjust accordingly for other concentrations. For µmol pack sizes, products should be diluted as 100µmol/ml to achieve 0.1M, regardless of molecular weight.

Item

Mol. Formula

Mol. Wt.

Unit Wt.

250mg

500mg

1g

LK2072 C44H54N7O9P 855.93 345.21 2.92 5.84 11.68

Coupling

No changes are required from the standard method recommended by the synthesiser manufacturer. Coupling is as per standard nucleoside amidites.

Deprotection

Cleave and deprotect with ammonium hydroxide containing 0.25M 2-mercaptoethanol for 17h at 55°C to avoid oxidative degradation of the 8-oxo-dG site.

Storage & Stability

Store in the fridge, dry. Stability in solution is 24h.

 

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