3'-Spacer C3 CPG
3'-Spacer C3 CPG
Key featuresShow Hide
- Incorporates a hydrophobic 3-carbon spacer at the 3' end of an oligonucleotide
- Useful as an alternative to 3'-phosphate for nuclease and polymerase resistance, especially with restriction enzymes
- Available in different pore sizes
- 500 Å CPG suitable for high yield applications such as therapeutic oligos (≤ 30mers).
- 1000 Å CPG suitable for highly modified oligonucleotides (> 20mers)
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In general terms, a spacer is introduced into an oligonucleotide to add distance between the oligonucleotide and a modifier. This reduces the possibility of any adverse interaction between the modifier and the sequence. For instance, G-rich sequences are known to quench fluorescein therefore the use of a suitable spacer will remove the dye label from the proximity of the oligonucleotide minimising the quenching effect. In a similar fashion, spacers are often used to distance between multiple additions of self-quenching dyes e.g. fluorescein.(1)
The application of the modified oligonucleotide will dictate whether a hydrophilic (Spacer 18 (HEG), Spacer 9 (TEG)) or hydrophobic spacer (Spacer C2, C3, C6, C12, C16) is required. Multiple incorporations of varying lengths of these spacers allow the precise length of the spacer arm to be controlled. This can be important in hairpin loop(2) and duplex studies(3) of DNA.
Several spacers have specific uses. A C3 spacer mimics the three carbon spacing between the 3’ and 5’ hydroxyls of a sugar unit.(4) Although useful where the base at a specific site is unknown, the flexibility of the alkyl chain distorts the sugar-phosphate backbone. This can be alleviated with the use of dSpacer since incorporation of this modifier sits directly into the natural sugar-phosphate backbone with no adverse effect. This modifier mimics abasic sites(5) and is useful in the study of mutations resulting from depurination.
Although less common than terminal spacing, but equally important, spacers have been incorporated within an oligonucleotide. This adds distance between sections of the sequence. For instance, Cytocell’s SMART detection assay(6) uses spacer 18 in the template probe where one section acts as an anchor in binding to the target leaving the other section free for hybridisation to the extension probe to allow amplification during PCR. In this case, the spacer gives flexibility to the template probe to enable hybridisation to both the target and the extension probe.
In similar way, spacer 18 is used in Scorpion™ Primers to separate the probe and primer section. However in this case, this not only provides the flexibility to allow the probe to flip back to hybridise to the amplicon but also acts as a PCR blocker to prevent read through to the probe.(7)
3'-Spacers are often used as an alternative to 3'-phosphate as blockers since, when incorporated at the 3'-end, the resulting oligonucleotide shows nuclease and polymerase resistance. In fact, spacer C3 is often incorporated at the 3'-end of an oligonucleotide for use with restriction enzymes rather than phosphate since the latter is thought to partially cleave during the assay.
- See for example: Design of multidye systems for FRET-based applications, M.S. Shchepinov and V.A. Korshun, Nucleosides, Nucleotides & Nucleic Acids, 20, 369-374, 2001.
- Circular dichroism studies of an oligodeoxyribonucleotide containing a hairpin loop made of a hexaethylene glycol chain: conformation and stability, M. Durand, K. Chevrie, M. Chassignol, N.T. Thuong and J.C. Maurizot, Nucleic Acids Research, 18, 6353-6359, 1990.
- A nicked duplex decamer DNA with a PEG6 tether, L. Kozerski, A.P. Mazurek, R. Kawecki, W. Bocian, P. Krajewski, E. Bednarek, J. Sitkowski, M. P. Williamson, A.J.G. Moir and P.E. Hansen, Nucleic Acids Research, 29, 1132-1143, 2001.
- Enhancing sequence-specific cleavage of RNA within a duplex region: Incorporation of 1,3-propanediol linkers into oligonucleotide conjugates of serinol-terpyridine, B.N. Trawick, T.A. Osiek and J.K. Bashkin, Bioconjugate Chem., 12, 900-905, 2001.
- (a) Oligodeoxynucleotides containing synthetic abasic sites model substrates for DNA-polymerases and apurinic apyrimidinic endonucleases, M. Takeshita, C.N. Chang, F. Johnson, S. Will and A.P. Grollman, J. Biol. Chem., 262, 10171-10179, 1987; (b) NMR-studies of abasic sites in DNA duplexes deoxyadenosine stacks into the helix opposite the cyclic analog of 2-deoxyribose, M.W. Kalnik, C.N. Chang, A.P. Grollman and D.J. Patel, Biochemistry, 27, 924-931, 1988.
- Detection of virus mRNA within infected host cells using an isothermal nucleic acid amplification assay: marine cyanophage gene expression within Synechococcus sp, S.D. Wharam, M.J. Hall and W.H. Wilson, Virology Journal, 4, 52-59, 2007.
- Duplex Scorpion primers in SNP analysis and FRET applications, A. Solinas, L.J. Brown, C. McKeen, J.M. Mellor, J.T.G. Nicol, N. Thelwell and T. Brown, Nucleic Acids Research, 29 (20), e96, 2001.
|LK2552||Spacer-CE Phosphoramidite C2|
|LK2113||Spacer-CE Phosphoramidite C3|
|LK2128||Spacer-CE Phosphoramidite 9|
|LK2129||Spacer-CE Phosphoramidite 18|
|LK2147||Spacer-CE Phosphoramidite C12|
|LK2245||3'-Spacer-C3 SynBase™ CPG 1000/110|
|LK2395||3'-Spacer-C3 SynBase™ CPG 3000/110|
Physical & Dilution Data
Dilution volumes (in ml) are for 0.1M solutions in dry acetonitrile (LK4050). Adjust accordingly for other concentrations. For µmol pack sizes, products should be diluted as 100µmol/ml to achieve 0.1M, regardless of molecular weight.
Prepare the amidite solutions 5-10min before use. It is recommended these are vortexed to ensure complete dissolution before placing on the synthesiser.
Coupling, Cleavage & Deprotection
For all spacer phosphoramidites, no changes are required from standard synthesiser protocols for either coupling. Likewise, standard oligonucleotide deprotection conditions can be applied when deprotecting an oligo containing these modifications.
The CPGs are used in a manner identical to standard protected nucleoside supports, however non-nucleosidic modification (using LK2245, LK2395 or LK2459) requires an initial detritylation prior to use in synthesis. For these modifications it is advised to carry out an initial deblock step, but not an initial capping step. This minimises the formation of N-1 product at the 3’ end. Cleavage and deprotection is achieved using the protocol required by the nucleobases. The cleavage and deprotection can also be done in one step by placing the resin in the deprotection solution then using the conditions required for deprotecting the nucleobases.
Storage & Stability
The products are stored dry in a freezer at –10 to –30°C. Phosphoramidite stability in solution is 2-3 days.