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O4-Chlorophenyl-U CE-Phosphoramidite

O4-Chlorophenyl-U CE-Phosphoramidite

CAS No.:220382-28-5

Convertible nucleoside phosphoramidite that allows the formation of N4-alkyl-C residues in RNA for structural studies.
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After incorporation of this unit into an oligoribonucleotide by standard phosphoramidite chemistry, treatment with ammonia, methylamine, or higher alkylamines, including those bearing tethered functional groups, leads to displacement of 4-chlorophenol with resultant installation of a 4-amino group, i.e., producing the desired N4-alkyl-C residues.(1)


  1. Allerson, C. R.; Chen, S. L.; Verdine, G. L. J. Am. Chem. Soc. 1997, 119, 7423-7433.

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