The formation and repair of alkylated DNA continues to be an important area of research. It has been shown that 3-methyl-2'-deoxycytidine (m3C) residues may be introduced site-specifically into DNA using the phosphoramidite 3-Methyl-dC-CE Phosphoramidite (BA 0282).⁽¹⁾

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1. See for example "Mutagenesis, genotoxicity, and repair of 1-methyladenine, 3-alkylcytosines, 1-methylguanine, and 3-methylthymine in alkB Escherichia coli", Delaney, J.; Essigmann, J. M., PNAS, 2004, 101s, 14051-14056. The supporting information of this paper includes the experimental details for the incorporation of m3C into oligonucleotides.